Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples
We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2006-12, Vol.44 (12), p.4572-4576 |
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| container_issue | 12 |
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| container_title | Journal of Clinical Microbiology |
| container_volume | 44 |
| creator | Jado, Isabel Escudero, Raquel Gil, Horacio Jiménez-Alonso, María Isabel Sousa, Rita García-Pérez, Ana L Rodríguez-Vargas, Manuela Lobo, Bruno Anda, Pedro |
| description | We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens. |
| doi_str_mv | 10.1128/JCM.01227-06 |
| format | Article |
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Psychology ; Humans ; Infectious diseases ; Medical sciences ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Phylogeny ; Polymerase Chain Reaction - methods ; Rickettsia ; Rickettsia - isolation & purification ; Rickettsia Infections - diagnosis ; Rickettsia Infections - microbiology ; Sensitivity and Specificity ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Siphonaptera - microbiology ; Ticks - microbiology ; Water Microbiology</subject><ispartof>Journal of Clinical Microbiology, 2006-12, Vol.44 (12), p.4572-4576</ispartof><rights>2007 INIST-CNRS</rights><rights>Copyright © 2006, American Society for Microbiology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-402f87de806d78afda92b8c73b459e27ae7b5401e782a9f3a6c837beaed719ba3</citedby><cites>FETCH-LOGICAL-c456t-402f87de806d78afda92b8c73b459e27ae7b5401e782a9f3a6c837beaed719ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1698399/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1698399/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3186,3187,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18366995$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17035495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jado, Isabel</creatorcontrib><creatorcontrib>Escudero, Raquel</creatorcontrib><creatorcontrib>Gil, Horacio</creatorcontrib><creatorcontrib>Jiménez-Alonso, María Isabel</creatorcontrib><creatorcontrib>Sousa, Rita</creatorcontrib><creatorcontrib>García-Pérez, Ana L</creatorcontrib><creatorcontrib>Rodríguez-Vargas, Manuela</creatorcontrib><creatorcontrib>Lobo, Bruno</creatorcontrib><creatorcontrib>Anda, Pedro</creatorcontrib><title>Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.</description><subject>Animals</subject><subject>Bacteriological Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chlamydiology and Rickettsiology</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Rickettsia</subject><subject>Rickettsia - isolation & purification</subject><subject>Rickettsia Infections - diagnosis</subject><subject>Rickettsia Infections - microbiology</subject><subject>Sensitivity and Specificity</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Siphonaptera - microbiology</subject><subject>Ticks - microbiology</subject><subject>Water Microbiology</subject><issn>0095-1137</issn><issn>1098-660X</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rFDEYhwdR7Fq9edZQ0FOn5mvycRHKUm2li-Ba8BbeyWR2U2eSNZmt-N-b7i5WT54Cb5734Zf8quolwWeEUPXu03xxhgmlssbiUTUjWKtaCPztcTXDWDc1IUweVc9yvsWYcN40T6sjIjFruG5mlVnEwdntAAkt3LSOHepjQledC5PvvYXJx4Bij754-91NU_aAlhtnvcvIBzQffCjQgCB06CLc-RTDWFbLZAnjZnD5efWkhyG7F4fzuLr5cPF1fllff_54NT-_ri1vxFRzTHslO6ew6KSCvgNNW2Ula3mjHZXgZNtwTJxUFHTPQFjFZOvAdZLoFthx9X7v3Wzb0XW2hEgwmE3yI6RfJoI3_94EvzareGeI0IppXQRvD4IUf2xdnszos3XDAMHFbTZCUVoSiP-CRDeEY3lvPN2DNsWck-v_pCHY3FdnSnVmV53ZeV_9_YIH-NBVAd4cAMjlz_sEwfr8wCkmhN5xJ3tu7Vfrnz45A3k0t3Y0nBtCDW8kLdDrPdRDNLBKRXSzpJgwXJJhLDT7DYQ3t5E</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Jado, Isabel</creator><creator>Escudero, Raquel</creator><creator>Gil, Horacio</creator><creator>Jiménez-Alonso, María Isabel</creator><creator>Sousa, Rita</creator><creator>García-Pérez, Ana L</creator><creator>Rodríguez-Vargas, Manuela</creator><creator>Lobo, Bruno</creator><creator>Anda, Pedro</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20061201</creationdate><title>Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples</title><author>Jado, Isabel ; Escudero, Raquel ; Gil, Horacio ; Jiménez-Alonso, María Isabel ; Sousa, Rita ; García-Pérez, Ana L ; Rodríguez-Vargas, Manuela ; Lobo, Bruno ; Anda, Pedro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-402f87de806d78afda92b8c73b459e27ae7b5401e782a9f3a6c837beaed719ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Bacteriological Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chlamydiology and Rickettsiology</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Rickettsia</topic><topic>Rickettsia - isolation & purification</topic><topic>Rickettsia Infections - diagnosis</topic><topic>Rickettsia Infections - microbiology</topic><topic>Sensitivity and Specificity</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Siphonaptera - microbiology</topic><topic>Ticks - microbiology</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jado, Isabel</creatorcontrib><creatorcontrib>Escudero, Raquel</creatorcontrib><creatorcontrib>Gil, Horacio</creatorcontrib><creatorcontrib>Jiménez-Alonso, María Isabel</creatorcontrib><creatorcontrib>Sousa, Rita</creatorcontrib><creatorcontrib>García-Pérez, Ana L</creatorcontrib><creatorcontrib>Rodríguez-Vargas, Manuela</creatorcontrib><creatorcontrib>Lobo, Bruno</creatorcontrib><creatorcontrib>Anda, Pedro</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jado, Isabel</au><au>Escudero, Raquel</au><au>Gil, Horacio</au><au>Jiménez-Alonso, María Isabel</au><au>Sousa, Rita</au><au>García-Pérez, Ana L</au><au>Rodríguez-Vargas, Manuela</au><au>Lobo, Bruno</au><au>Anda, Pedro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2006-12-01</date><risdate>2006</risdate><volume>44</volume><issue>12</issue><spage>4572</spage><epage>4576</epage><pages>4572-4576</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><eissn>1098-5530</eissn><coden>JCMIDW</coden><abstract>We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. 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| subjects | Animals Bacteriological Techniques Bacteriology Biological and medical sciences Chlamydiology and Rickettsiology DNA, Bacterial - genetics DNA, Ribosomal Spacer - genetics Fundamental and applied biological sciences. Psychology Humans Infectious diseases Medical sciences Microbiology Miscellaneous Molecular Sequence Data Nucleic Acid Hybridization Phylogeny Polymerase Chain Reaction - methods Rickettsia Rickettsia - isolation & purification Rickettsia Infections - diagnosis Rickettsia Infections - microbiology Sensitivity and Specificity Sequence Analysis, DNA Sequence Homology, Nucleic Acid Siphonaptera - microbiology Ticks - microbiology Water Microbiology |
| title | Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples |
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