Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples

We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental...

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Veröffentlicht in:	Journal of Clinical Microbiology 2006-12, Vol.44 (12), p.4572-4576
Hauptverfasser:	Jado, Isabel, Escudero, Raquel, Gil, Horacio, Jiménez-Alonso, María Isabel, Sousa, Rita, García-Pérez, Ana L, Rodríguez-Vargas, Manuela, Lobo, Bruno, Anda, Pedro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriological Techniques Bacteriology Biological and medical sciences Chlamydiology and Rickettsiology DNA, Bacterial - genetics DNA, Ribosomal Spacer - genetics Fundamental and applied biological sciences. Psychology Humans Infectious diseases Medical sciences Microbiology Miscellaneous Molecular Sequence Data Nucleic Acid Hybridization Phylogeny Polymerase Chain Reaction - methods Rickettsia Rickettsia - isolation & purification Rickettsia Infections - diagnosis Rickettsia Infections - microbiology Sensitivity and Specificity Sequence Analysis, DNA Sequence Homology, Nucleic Acid Siphonaptera - microbiology Ticks - microbiology Water Microbiology
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Beschreibung
Zusammenfassung:	We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.
ISSN:	0095-1137 1098-660X 1098-5530
DOI:	10.1128/JCM.01227-06