Defects in Conidiophore Development and Conidium-Macrophage Interactions in a Dioxygenase Mutant of Aspergillus fumigatus
Oxygenated fatty acids, or oxylipins, play an essential role in physiological signaling and developmental processes in animals, plants, and fungi. Previous characterization of three Aspergillus fumigatus dioxygenases (PpoA, PpoB, and PpoC), similar in sequence to mammalian cyclooxygenases, showed th...
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| Veröffentlicht in: | Infection and Immunity 2008-07, Vol.76 (7), p.3214-3220 |
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| creator | Dagenais, Taylor R.T Chung, DaWoon Giles, Steven S Hull, Christina M Andes, David Keller, Nancy P |
| description | Oxygenated fatty acids, or oxylipins, play an essential role in physiological signaling and developmental processes in animals, plants, and fungi. Previous characterization of three Aspergillus fumigatus dioxygenases (PpoA, PpoB, and PpoC), similar in sequence to mammalian cyclooxygenases, showed that PpoA is responsible for the production of the oxylipins 8R-hydroperoxyoctadecadienoic acid and 5S,8R-dihydroxy-9Z,12Z-octadecadienoic acid and that PpoC is responsible for 10R-hydroxy-8E,12Z-hydroperoxyoctadecadienoic acid. Here, Δppo mutants were characterized to elucidate the role of fungal dioxygenases in A. fumigatus development and host interactions. The ΔppoC strain displayed distinct phenotypes compared to those of other Δppo mutants and the wild type, including altered conidium size, germination, and tolerance to oxidative stress as well as increased uptake and killing by primary alveolar macrophages. These experiments implicate oxylipins in pathogen development and suggest that ΔppoC represents a useful model for studying the A. fumigatus-host interaction. |
| doi_str_mv | 10.1128/IAI.00009-08 |
| format | Article |
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Previous characterization of three Aspergillus fumigatus dioxygenases (PpoA, PpoB, and PpoC), similar in sequence to mammalian cyclooxygenases, showed that PpoA is responsible for the production of the oxylipins 8R-hydroperoxyoctadecadienoic acid and 5S,8R-dihydroxy-9Z,12Z-octadecadienoic acid and that PpoC is responsible for 10R-hydroxy-8E,12Z-hydroperoxyoctadecadienoic acid. Here, Δppo mutants were characterized to elucidate the role of fungal dioxygenases in A. fumigatus development and host interactions. The ΔppoC strain displayed distinct phenotypes compared to those of other Δppo mutants and the wild type, including altered conidium size, germination, and tolerance to oxidative stress as well as increased uptake and killing by primary alveolar macrophages. These experiments implicate oxylipins in pathogen development and suggest that ΔppoC represents a useful model for studying the A. fumigatus-host interaction.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.00009-08</identifier><identifier>PMID: 18443090</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Animals, Outbred Strains ; Aspergillosis - microbiology ; Aspergillus fumigatus ; Aspergillus fumigatus - enzymology ; Aspergillus fumigatus - genetics ; Aspergillus fumigatus - pathogenicity ; Aspergillus fumigatus - physiology ; Biological and medical sciences ; Dioxygenases - genetics ; Dioxygenases - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Fungal and Parasitic Infections ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Host-Pathogen Interactions ; Macrophages, Alveolar - immunology ; Macrophages, Alveolar - microbiology ; Mice ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Microbiology ; Miscellaneous ; Mutation ; Mycology ; Oxidative Stress ; Phagocytosis ; Spores, Fungal - physiology ; Virulence</subject><ispartof>Infection and Immunity, 2008-07, Vol.76 (7), p.3214-3220</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright © 2008, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-82f2a7cfb0554373dc78274f4292985a4f00c6e8ead7a293a9f1217f6aaf25cd3</citedby><cites>FETCH-LOGICAL-c493t-82f2a7cfb0554373dc78274f4292985a4f00c6e8ead7a293a9f1217f6aaf25cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446696/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446696/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20460182$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18443090$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dagenais, Taylor R.T</creatorcontrib><creatorcontrib>Chung, DaWoon</creatorcontrib><creatorcontrib>Giles, Steven S</creatorcontrib><creatorcontrib>Hull, Christina M</creatorcontrib><creatorcontrib>Andes, David</creatorcontrib><creatorcontrib>Keller, Nancy P</creatorcontrib><title>Defects in Conidiophore Development and Conidium-Macrophage Interactions in a Dioxygenase Mutant of Aspergillus fumigatus</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>Oxygenated fatty acids, or oxylipins, play an essential role in physiological signaling and developmental processes in animals, plants, and fungi. Previous characterization of three Aspergillus fumigatus dioxygenases (PpoA, PpoB, and PpoC), similar in sequence to mammalian cyclooxygenases, showed that PpoA is responsible for the production of the oxylipins 8R-hydroperoxyoctadecadienoic acid and 5S,8R-dihydroxy-9Z,12Z-octadecadienoic acid and that PpoC is responsible for 10R-hydroxy-8E,12Z-hydroperoxyoctadecadienoic acid. Here, Δppo mutants were characterized to elucidate the role of fungal dioxygenases in A. fumigatus development and host interactions. The ΔppoC strain displayed distinct phenotypes compared to those of other Δppo mutants and the wild type, including altered conidium size, germination, and tolerance to oxidative stress as well as increased uptake and killing by primary alveolar macrophages. These experiments implicate oxylipins in pathogen development and suggest that ΔppoC represents a useful model for studying the A. fumigatus-host interaction.</description><subject>Animals</subject><subject>Animals, Outbred Strains</subject><subject>Aspergillosis - microbiology</subject><subject>Aspergillus fumigatus</subject><subject>Aspergillus fumigatus - enzymology</subject><subject>Aspergillus fumigatus - genetics</subject><subject>Aspergillus fumigatus - pathogenicity</subject><subject>Aspergillus fumigatus - physiology</subject><subject>Biological and medical sciences</subject><subject>Dioxygenases - genetics</subject><subject>Dioxygenases - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal and Parasitic Infections</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Host-Pathogen Interactions</subject><subject>Macrophages, Alveolar - immunology</subject><subject>Macrophages, Alveolar - microbiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred ICR</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mutation</subject><subject>Mycology</subject><subject>Oxidative Stress</subject><subject>Phagocytosis</subject><subject>Spores, Fungal - physiology</subject><subject>Virulence</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhSMEotPCjjWEBaxI8SuxvUEazfAYqRUL6Nq6deyMUWIHOynMv6_biQqs8May7ueje84pihcYnWNMxPvdeneO8pEVEo-KFUZSVHVNyONihRCWlawbflKcpvQjPxlj4mlxggVjFEm0Kg5bY42eUul8uQnetS6M-xBNuTU3pg_jYPxUgm-X4TxUl6BjZqAz5c5PJoKeXPD3AlBuXfh96IyHZMrLeYL8OdhynUYTO9f3cyrtPLgOpjk9K55Y6JN5vtxnxdWnj983X6qLr593m_VFpZmkUyWIJcC1vUZ1zSinreaCcGYZkUSKGphFSDdGGGg5EElBWkwwtw2AJbVu6Vnx4ag7zteDaXU2FKFXY3QDxIMK4NS_E-_2qgs3ijDWNLLJAm8XgRh-ziZNanBJm74Hb8KcVCMJpSgH-j8QS8aplDKD745gTjKlaOzDNhipu1JVLlXdl6qQyPjLvx38gZcWM_BmASBp6G0Er1164AhiDcKCZO71kdu7bv_LRaMgDcrlBHijuKIE35l4dWQsBAVdzDpX3wjCNC-DOJeM3gKW1sC_</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Dagenais, Taylor R.T</creator><creator>Chung, DaWoon</creator><creator>Giles, Steven S</creator><creator>Hull, Christina M</creator><creator>Andes, David</creator><creator>Keller, Nancy P</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080701</creationdate><title>Defects in Conidiophore Development and Conidium-Macrophage Interactions in a Dioxygenase Mutant of Aspergillus fumigatus</title><author>Dagenais, Taylor R.T ; Chung, DaWoon ; Giles, Steven S ; Hull, Christina M ; Andes, David ; Keller, Nancy P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-82f2a7cfb0554373dc78274f4292985a4f00c6e8ead7a293a9f1217f6aaf25cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Animals, Outbred Strains</topic><topic>Aspergillosis - microbiology</topic><topic>Aspergillus fumigatus</topic><topic>Aspergillus fumigatus - enzymology</topic><topic>Aspergillus fumigatus - genetics</topic><topic>Aspergillus fumigatus - pathogenicity</topic><topic>Aspergillus fumigatus - physiology</topic><topic>Biological and medical sciences</topic><topic>Dioxygenases - genetics</topic><topic>Dioxygenases - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal and Parasitic Infections</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Host-Pathogen Interactions</topic><topic>Macrophages, Alveolar - immunology</topic><topic>Macrophages, Alveolar - microbiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred ICR</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mutation</topic><topic>Mycology</topic><topic>Oxidative Stress</topic><topic>Phagocytosis</topic><topic>Spores, Fungal - physiology</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dagenais, Taylor R.T</creatorcontrib><creatorcontrib>Chung, DaWoon</creatorcontrib><creatorcontrib>Giles, Steven S</creatorcontrib><creatorcontrib>Hull, Christina M</creatorcontrib><creatorcontrib>Andes, David</creatorcontrib><creatorcontrib>Keller, Nancy P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and Immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dagenais, Taylor R.T</au><au>Chung, DaWoon</au><au>Giles, Steven S</au><au>Hull, Christina M</au><au>Andes, David</au><au>Keller, Nancy P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Defects in Conidiophore Development and Conidium-Macrophage Interactions in a Dioxygenase Mutant of Aspergillus fumigatus</atitle><jtitle>Infection and Immunity</jtitle><addtitle>Infect Immun</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>76</volume><issue>7</issue><spage>3214</spage><epage>3220</epage><pages>3214-3220</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>Oxygenated fatty acids, or oxylipins, play an essential role in physiological signaling and developmental processes in animals, plants, and fungi. Previous characterization of three Aspergillus fumigatus dioxygenases (PpoA, PpoB, and PpoC), similar in sequence to mammalian cyclooxygenases, showed that PpoA is responsible for the production of the oxylipins 8R-hydroperoxyoctadecadienoic acid and 5S,8R-dihydroxy-9Z,12Z-octadecadienoic acid and that PpoC is responsible for 10R-hydroxy-8E,12Z-hydroperoxyoctadecadienoic acid. Here, Δppo mutants were characterized to elucidate the role of fungal dioxygenases in A. fumigatus development and host interactions. The ΔppoC strain displayed distinct phenotypes compared to those of other Δppo mutants and the wild type, including altered conidium size, germination, and tolerance to oxidative stress as well as increased uptake and killing by primary alveolar macrophages. These experiments implicate oxylipins in pathogen development and suggest that ΔppoC represents a useful model for studying the A. fumigatus-host interaction.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>18443090</pmid><doi>10.1128/IAI.00009-08</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Outbred Strains Aspergillosis - microbiology Aspergillus fumigatus Aspergillus fumigatus - enzymology Aspergillus fumigatus - genetics Aspergillus fumigatus - pathogenicity Aspergillus fumigatus - physiology Biological and medical sciences Dioxygenases - genetics Dioxygenases - metabolism Female Fundamental and applied biological sciences. Psychology Fungal and Parasitic Infections Fungal Proteins - genetics Fungal Proteins - metabolism Host-Pathogen Interactions Macrophages, Alveolar - immunology Macrophages, Alveolar - microbiology Mice Mice, Inbred C57BL Mice, Inbred ICR Microbiology Miscellaneous Mutation Mycology Oxidative Stress Phagocytosis Spores, Fungal - physiology Virulence |
| title | Defects in Conidiophore Development and Conidium-Macrophage Interactions in a Dioxygenase Mutant of Aspergillus fumigatus |
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