The Effect of Blueberry Extracts and Quercetin on Capacitation Status of Stored Boar Sperm

Liquid cooled boar semen is highly susceptible to lipid peroxidation and capacitation in vitro during storage, which results in reduced fertility and litter sizes following artificial insemination (Al). Antioxidants can delay lipid peroxidation in vitro and therefore potentially extend the lifespan of stored semen. Lowbush blueberries (Vaccinium angustifolium Aiton) and their leaves contain high antioxidant capacity, making them potential candidates for preserving liquid cooled boar semen during storage. This study compared the reversible ability of blueberry extract, leaf extract, and the antioxidant quercetin to prevent capacitation of extended, cooled boar semen during storage for 1 week. The results indicated that blueberry leaf extract and quercetin were equally capable of delaying capacitation for 1 week and still allow capacitation to occur in vitro following incubation at 39 degree C. Blueberry extract also delayed capacitation but only during storage for 5 days; its effects were also reversible. Blueberry leaf extract and quercetin are potential supplements for storage of liquid cooled boar semen.