Design and testing of a 750MHz CW-EPR digital console for small animal imaging

[Display omitted] •A digital console for 3D CW-EPR imaging using band-pass subsampling.•Automatic control to keep a resonator under critical coupling conditions.•In vivo 3D EPR imaging of nitroxyl radicals in a mouse’s head within 10s. This paper describes the development of a digital console for three-dimensional (3D) continuous wave electron paramagnetic resonance (CW-EPR) imaging of a small animal to improve the signal-to-noise ratio and lower the cost of the EPR imaging system. A RF generation board, an RF acquisition board and a digital signal processing (DSP) & control board were built for the digital EPR detection. Direct sampling of the reflected RF signal from a resonator (approximately 750MHz), which contains the EPR signal, was carried out using a band-pass subsampling method. A direct automatic control system to reduce the reflection from the resonator was proposed and implemented in the digital EPR detection scheme. All DSP tasks were carried out in field programmable gate array ICs. In vivo 3D imaging of nitroxyl radicals in a mouse’s head was successfully performed.