Molecular mapping and identification of QTL's associated to oat crown rust partial resistance

Molecular mapping is a promising strategy for studying and understanding traits with complex genetic control, such as partial resistance to oat crown rust. The objectives of this research were to develop molecular maps from the progenies of the cross UFRGS7 (susceptible) × UFRGS910906 (partially resistant) and to identify QTLs (quantitative trait loci) associated to partial resistance to oat crown rust in two generations of that population.DNA of 86 genotypes of the F^sub 2^ and 90 genotypes of the F^sub 6^ UFRGS7 × UFRGS910906 population were used to generate AFLP markers. Molecular maps were constructed using Mapmaker Exp. 3.0 and QTLs for partial resistance to oat crown rust were identified with Mapmaker/QTL software. Five hundred and fifty seven markers in the F^sub 2^ and 243 markers in the F^sub 6^ generations were identified. The F^sub 2^ map integrated 250 markers in 37 linkage groups. The F^sub 6^ map integrated 86 markers in 17 linkage groups.Five QTLs were identified for partial resistance to oat crown rust in the F^sub 2^ generation and three QTLs in the F^sub 6^. The QTL identified on F^sub 6^ through the PaaaMctt340 AFLP marker showed consistency across two environments and two generations (F^sub 4^ and F^sub 6^), and appear to have potential for marker-assisted selection in oat.[PUBLICATION ABSTRACT]