The Contribution of TWIK-Related Acid-Sensitive K super(+)-Containing Channels to the Function of Dorsal Lateral Geniculate Thalamocortical Relay Neurons

A genetic knockout was used to determine the specific contribution of TWIK-related acid-sensitive K super(+) (TASK)-1 channels to the function of dorsal lateral geniculate nucleus (DLG) thalamocortical relay (TC) neurons. Disruption of TASK-1 function produced an similar to 19% decrease in amplitude of the standing outward current (I sub(SO)) and a 3 plus or minus 1-mV depolarizing shift in resting membrane potential (V sub(rest)) of DLG neurons. We estimated that current through TASK-1 homodimers or TASK-1/TASK-3 heterodimers contribute(s) approximately one third of the current sensitive to TASK channel modulators in DLG TC neurons. The effects of the TASK channel blocker bupivacaine (20 mu M), of muscarine (50 mu M), and of H super(+) on I sub(SO) were reduced to approximately 60%, 59%, and shifted to more acidic pH values, respectively. The blocking effect of anandamide on I sub(SO) [30 mu M; 23 plus or minus 3% current decrease in wild type (WT)] was absent in TASK-1 knockout (TASK-1 super(-/-)) mice (9 plus or minus 6% current increase). Comparable results were obtained with the more stable anand-amide derivative methanandamide (20 mu M; 20 plus or minus 2% decrease in WT; 4 plus or minus 6% increase in TASK-1 super(-/-)). Current-clamp recordings revealed a muscarine-induced shift in TC neuron activity from burst to tonic firing in both mouse genotypes. Electrocorticograms and sleep/wake times were unchanged in TASK-1 super(-/-) mice. In conclusion, our findings demonstrate a significant contribution of TASK-1 channels to I sub(SO) in DLG TC neurons, although the genetic knockout of TASK-1 did not produce severe deficits in the thalamocortical system.