Sexual behavior in ladybird beetles: Sex with lights on and a twist for Tenuisvalvae notata (Coleoptera: Coccinellidae)

•Sexual activity of T. notata is diurnal with peak between 1100h and 1500h.•Females start mating at emergence, whereas males wait an average of 4days to it.•Adults mate multiple times, and each copulation last about 84s.•Males twist on the females’ back after a conspicuous copulation. The ladybird beetle Tenuisvalvae notata is an important predator of mealybugs (Pseudococcidae); however, little is known about its reproductive behavior. Thus, in order to improve methods of its rearing, this work studied several aspects regarding the sexual behavior of T. notata. We investigated its sexual activity period, age of the first copulation, mating frequency over 24h, and oviposition during a 30-day interval. Sexual activity of T. notata is diurnal with peak between 1100h and 1500h. Males need about 4days to first copulation, whereas females can mate at emergence. Adults mate 1.17±0.16–1.91±0.29 times over 24h with an average duration of 84±19.70s (ranging from 27 to 130s) per mating. Females produced an average of 54±6.42–64±7.08 offspring over 30days. An ethogram was also constructed to depict the events that occurred during copulation as follows: the male mounts the female, inserts the aedeagus, touches its back with palps and mandibles, and attempts to hold it simultaneously. The female can walk while copulating or remains motionless; the male retracts its aedeagus and twists on the females’ back before moving apart.