Application of cation-exchange membranes for characterisation and imaging ammonia-oxidising bacteria in soils

A new approach, in which ammonia-oxidizing bacteria (AOB) are entrapped from soil onto cation-exchange membranes, was applied to identify terrestrial AOB by fluorescence in situ hybridization (FISH). An experimental hot spot of ammonia oxidation was developed by establishing a gradient of ammonium s...

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Veröffentlicht in:	FEMS microbiology ecology 2005-08, Vol.53 (3), p.463-472
Hauptverfasser:	Kurola, Jukka, Wittmann, Christoph, Salkinoja-Salonen, Mirja, Aarnio, Tuula, Romantschuk, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	16S rRNA gene Ammonia Ammonia - metabolism Ammonia-oxidizing bacteria Ammonium Bacteria Bacteriology Betaproteobacteria - classification Betaproteobacteria - genetics Betaproteobacteria - isolation & purification Betaproteobacteria - metabolism Biological and medical sciences Cation Exchange Resins Cation exchanging Cation-exchange membrane Colony Count, Microbial DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Ecology Finland Fish oils Fluorescence Fluorescence in situ hybridization Fundamental and applied biological sciences. Psychology Gene sequencing In Situ Hybridization, Fluorescence Industrial Waste Membranes Membranes, Artificial Microbiology Miscellaneous Nitrosospira Oil pollution Oxidation Oxidation-Reduction Petroleum RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, DNA Soil bacteria Soil Microbiology Soil microorganisms Soil Pollutants - metabolism Soil pollution Soils Substrates
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Zusammenfassung:	A new approach, in which ammonia-oxidizing bacteria (AOB) are entrapped from soil onto cation-exchange membranes, was applied to identify terrestrial AOB by fluorescence in situ hybridization (FISH). An experimental hot spot of ammonia oxidation was developed by establishing a gradient of ammonium substrate (200 to
ISSN:	0168-6496 1574-6941
DOI:	10.1016/j.femsec.2005.02.001