Low-cost mass culture method using the commercial diatom Chaetoceros neogracile as an original strain seed during winter

The aim of this study was to examine the short-term expansion culture of the commercial diatom Chaetoceros neogracile as an original strain seed during winter. Water temperature, diatom cell density, illumination intensity, time, and handling stress during pre-treatment were analyzed as explanatory variables that could affect the specific growth rates of the diatom during culture, using linear mixed-effects models. The results indicated that water temperature was the only significant explanatory factor (p