Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR
In the study 251 fresh ground beef samples sold in Ankara were analyzed to evaluate the prevalence of Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef sample...
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| Veröffentlicht in: | Food control 2009-04, Vol.20 (4), p.357-361 |
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| creator | Sarimehmetoglu, Belgin Aksoy, Mihriban Hatun Ayaz, Naim Deniz Ayaz, Yildiz Kuplulu, Ozlem Kaplan, Yusuf Ziya |
| description | In the study 251 fresh ground beef samples sold in Ankara were analyzed to evaluate the prevalence of
Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef samples were found as contaminated with
E. coli O157. By PCR analyse in two of the isolates
fliC
h
7 gene was detected and identified as
E. coli O157:H7. According to the multiplex PCR one of the isolate has all
stx
1,
stx
2,
eaeA,
hly and
fliC
h
7 genes and the other has
stx
1,
eaeA,
hly and
fliC
h
7 genes. |
| doi_str_mv | 10.1016/j.foodcont.2008.06.002 |
| format | Article |
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Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef samples were found as contaminated with
E. coli O157. By PCR analyse in two of the isolates
fliC
h
7 gene was detected and identified as
E. coli O157:H7. According to the multiplex PCR one of the isolate has all
stx
1,
stx
2,
eaeA,
hly and
fliC
h
7 genes and the other has
stx
1,
eaeA,
hly and
fliC
h
7 genes.</description><identifier>ISSN: 0956-7135</identifier><identifier>EISSN: 1873-7129</identifier><identifier>DOI: 10.1016/j.foodcont.2008.06.002</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>bacterial contamination ; beef quality ; E. coli O157:H7 ; Escherichia coli ; Escherichia coli O157:H7 ; fliCh7 gene ; food analysis ; food contamination ; genes ; Ground beef ; immunomagnetic separation ; meat composition ; microbial detection ; Multiplex PCR ; multiplex polymerase chain reaction ; polymerase chain reaction ; virulence</subject><ispartof>Food control, 2009-04, Vol.20 (4), p.357-361</ispartof><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-c5bb8a6ff9c233d4ecd2aebb6dafe43c5b478e088ef1ffd76d79d94dd2fac5713</citedby><cites>FETCH-LOGICAL-c433t-c5bb8a6ff9c233d4ecd2aebb6dafe43c5b478e088ef1ffd76d79d94dd2fac5713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.foodcont.2008.06.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Sarimehmetoglu, Belgin</creatorcontrib><creatorcontrib>Aksoy, Mihriban Hatun</creatorcontrib><creatorcontrib>Ayaz, Naim Deniz</creatorcontrib><creatorcontrib>Ayaz, Yildiz</creatorcontrib><creatorcontrib>Kuplulu, Ozlem</creatorcontrib><creatorcontrib>Kaplan, Yusuf Ziya</creatorcontrib><title>Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR</title><title>Food control</title><description>In the study 251 fresh ground beef samples sold in Ankara were analyzed to evaluate the prevalence of
Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef samples were found as contaminated with
E. coli O157. By PCR analyse in two of the isolates
fliC
h
7 gene was detected and identified as
E. coli O157:H7. According to the multiplex PCR one of the isolate has all
stx
1,
stx
2,
eaeA,
hly and
fliC
h
7 genes and the other has
stx
1,
eaeA,
hly and
fliC
h
7 genes.</description><subject>bacterial contamination</subject><subject>beef quality</subject><subject>E. coli O157:H7</subject><subject>Escherichia coli</subject><subject>Escherichia coli O157:H7</subject><subject>fliCh7 gene</subject><subject>food analysis</subject><subject>food contamination</subject><subject>genes</subject><subject>Ground beef</subject><subject>immunomagnetic separation</subject><subject>meat composition</subject><subject>microbial detection</subject><subject>Multiplex PCR</subject><subject>multiplex polymerase chain reaction</subject><subject>polymerase chain reaction</subject><subject>virulence</subject><issn>0956-7135</issn><issn>1873-7129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkE1P3DAQhi0EEgv0L4BPvW06jhMn6alo-aqEtIiWs-XY48WrxF7spCr_vt5ue-Y0I80zM3ofQi4ZFAyY-LItbAhGBz8VJUBbgCgAyiOyYG3Dlw0ru2OygK4Wuef1KTlLaQvAGmCwIPYGJ9STC54GS2-TfsXo9KtTVIfB0TWrm68PDXWebmKYvaE9oqVzcn5D3TjOPoxq43Fymibcqaj-nlIZHOdhcrsBf9On1fMFObFqSPjpXz0nL3e3P1cPy8f1_ffV9eNSV5xPS133fauEtZ0uOTcValMq7HthlMWK53HVtAhti5ZZaxphms50lTGlVbrO8c7J58PdXQxvM6ZJji5pHAblMcxJso530EKdQXEAdQwpRbRyF92o4rtkIPda5Vb-1yr3WiUImbXmxavDolVBqk10Sb78KIFxYLXgreCZ-HYgMAf95TDKpB16jcbFrFqa4D568gcXy4_d</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Sarimehmetoglu, Belgin</creator><creator>Aksoy, Mihriban Hatun</creator><creator>Ayaz, Naim Deniz</creator><creator>Ayaz, Yildiz</creator><creator>Kuplulu, Ozlem</creator><creator>Kaplan, Yusuf Ziya</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20090401</creationdate><title>Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR</title><author>Sarimehmetoglu, Belgin ; Aksoy, Mihriban Hatun ; Ayaz, Naim Deniz ; Ayaz, Yildiz ; Kuplulu, Ozlem ; Kaplan, Yusuf Ziya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-c5bb8a6ff9c233d4ecd2aebb6dafe43c5b478e088ef1ffd76d79d94dd2fac5713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>bacterial contamination</topic><topic>beef quality</topic><topic>E. coli O157:H7</topic><topic>Escherichia coli</topic><topic>Escherichia coli O157:H7</topic><topic>fliCh7 gene</topic><topic>food analysis</topic><topic>food contamination</topic><topic>genes</topic><topic>Ground beef</topic><topic>immunomagnetic separation</topic><topic>meat composition</topic><topic>microbial detection</topic><topic>Multiplex PCR</topic><topic>multiplex polymerase chain reaction</topic><topic>polymerase chain reaction</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarimehmetoglu, Belgin</creatorcontrib><creatorcontrib>Aksoy, Mihriban Hatun</creatorcontrib><creatorcontrib>Ayaz, Naim Deniz</creatorcontrib><creatorcontrib>Ayaz, Yildiz</creatorcontrib><creatorcontrib>Kuplulu, Ozlem</creatorcontrib><creatorcontrib>Kaplan, Yusuf Ziya</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food control</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarimehmetoglu, Belgin</au><au>Aksoy, Mihriban Hatun</au><au>Ayaz, Naim Deniz</au><au>Ayaz, Yildiz</au><au>Kuplulu, Ozlem</au><au>Kaplan, Yusuf Ziya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR</atitle><jtitle>Food control</jtitle><date>2009-04-01</date><risdate>2009</risdate><volume>20</volume><issue>4</issue><spage>357</spage><epage>361</epage><pages>357-361</pages><issn>0956-7135</issn><eissn>1873-7129</eissn><abstract>In the study 251 fresh ground beef samples sold in Ankara were analyzed to evaluate the prevalence of
Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef samples were found as contaminated with
E. coli O157. By PCR analyse in two of the isolates
fliC
h
7 gene was detected and identified as
E. coli O157:H7. According to the multiplex PCR one of the isolate has all
stx
1,
stx
2,
eaeA,
hly and
fliC
h
7 genes and the other has
stx
1,
eaeA,
hly and
fliC
h
7 genes.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.foodcont.2008.06.002</doi><tpages>5</tpages></addata></record> |
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| ispartof | Food control, 2009-04, Vol.20 (4), p.357-361 |
| issn | 0956-7135 1873-7129 |
| language | eng |
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| source | Access via ScienceDirect (Elsevier) |
| subjects | bacterial contamination beef quality E. coli O157:H7 Escherichia coli Escherichia coli O157:H7 fliCh7 gene food analysis food contamination genes Ground beef immunomagnetic separation meat composition microbial detection Multiplex PCR multiplex polymerase chain reaction polymerase chain reaction virulence |
| title | Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR |
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