Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR

In the study 251 fresh ground beef samples sold in Ankara were analyzed to evaluate the prevalence of Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef sample...

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Veröffentlicht in:	Food control 2009-04, Vol.20 (4), p.357-361
Hauptverfasser:	Sarimehmetoglu, Belgin, Aksoy, Mihriban Hatun, Ayaz, Naim Deniz, Ayaz, Yildiz, Kuplulu, Ozlem, Kaplan, Yusuf Ziya
Format:	Artikel
Sprache:	eng
Schlagworte:	bacterial contamination beef quality E. coli O157:H7 Escherichia coli Escherichia coli O157:H7 fliCh7 gene food analysis food contamination genes Ground beef immunomagnetic separation meat composition microbial detection Multiplex PCR multiplex polymerase chain reaction polymerase chain reaction virulence
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Beschreibung
Zusammenfassung:	In the study 251 fresh ground beef samples sold in Ankara were analyzed to evaluate the prevalence of Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef samples were found as contaminated with E. coli O157. By PCR analyse in two of the isolates fliC h 7 gene was detected and identified as E. coli O157:H7. According to the multiplex PCR one of the isolate has all stx 1, stx 2, eaeA, hly and fliC h 7 genes and the other has stx 1, eaeA, hly and fliC h 7 genes.
ISSN:	0956-7135 1873-7129
DOI:	10.1016/j.foodcont.2008.06.002