Structure of SAP18: A Ubiquitin Fold in Histone Deacetylase Complex Assembly

Signal transduction pathways are frequently found to repress transcription of target genes in the absence of stimulation and, conversely, to upregulate transcription in the presence of a signal. Transcription factors are central in this dual regulatory mechanism and widely use a generalized mechanism to repress transcription through recruitment of a Sin3-histone deacetylase (HDAC) complex to their binding sites on DNA. The protein SAP 18 (Sin3-associated polypeptide of 18 kDa) has been shown to play a key role in gene-specific recruitment of the HDAC complex by a number of transcription factors including Gli, GAGA, and Bicoid. The solution structure of SAP 18 reveals a ubiquitin-like fold with several large loop insertions relative to other family members. This fold supports the functional role of SAP 18 as a protein-protein adapter module and provides insight for how SAP 18 may bridge the Sin3-HDAC complex to transcription factors.