MCPIP1 attenuates the innate immune response to influenza A virus by suppressing RIG‐I expression in lung epithelial cells

The pattern recognition receptor retinoic acid‐inducible gene I (RIG‐I) reportedly plays a key role in sensing influenza A virus (IAV) infection and activating type I interferon (IFN) response. MCP‐1‐induced protein 1 (MCPIP1) can directly degrade cytokine mRNAs, such as IL‐6, IL‐12, IL‐1β, and IL‐2, by functioning as an RNase. Here, we initially observed that MCPIP1 exhibited virus supportive functions later in the course of IAV infection in A549 cells, and negatively regulated IAV‐induced RIG‐I‐dependent innate antiviral response. Exogenous overexpression of MCPIP1 suppressed the expression of RIG‐I, whereas shRNA‐mediated inhibition of endogenous MCPIP1 enhanced RIG‐I expression. The results of experiments with actinomycin D and luciferase assay demonstrated that MCPIP1 reduced RIG‐I expression through destabilizing its mRNA. Various mutants of functional domains of MCPIP1 further confirmed that the inhibitory effect of MCPIP1 on RIG‐I expression required RNase activity but not deubiquitinase activity. Finally, the overexpression of several IAV proteins, which have the ability to inhibit the host IFN response at different levels, induced MCPIP1 expression, especially non‐structural protein 1 (NS1). Conclusively, these data demonstrate the MCPIP1 contributes to attenuate IAV‐induced host antiviral response by suppressing RIG‐I expression.