Strategies to improve beef tenderness by activating calpain-2 earlier postmortem

Our objectives were to determine the effect of post rigor calcium chloride injection or freezing on 1) sarcoplasmic calcium concentration and calpain-2 activity of beef longissimus lumborum (LL) and semimembranosus (SM) steaks aged 1, 4, and 14days post-treatment and on 2) Warner-Bratzler shear forc...

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Veröffentlicht in:Meat science 2018-01, Vol.135, p.36-41
Hauptverfasser: Colle, M.J., Nasados, J.A., Rogers, J.M., Kerby, D.M., Colle, M.M., Van Buren, J.B., Richard, R.P., Murdoch, G.K., Williams, C.J., Doumit, M.E.
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Sprache:eng
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Zusammenfassung:Our objectives were to determine the effect of post rigor calcium chloride injection or freezing on 1) sarcoplasmic calcium concentration and calpain-2 activity of beef longissimus lumborum (LL) and semimembranosus (SM) steaks aged 1, 4, and 14days post-treatment and on 2) Warner-Bratzler shear force, water holding capacity, and consumer acceptability of LL and SM steaks aged 4 and 14days post-treatment. Free calcium levels in the calcium, frozen, and control steaks averaged 1256, 127, and 121μM for the LL and 1520, 120, and 111μM for the SM, respectively. Measurable LL native calpain-2 activity was lower in calcium and frozen steaks than control steaks (P
ISSN:0309-1740
1873-4138
DOI:10.1016/j.meatsci.2017.08.008