Enhancing recovery of recombinant hepatitis B surface antigen in lab-scale and large-scale anion-exchange chromatography by optimizing the conductivity of buffers

In biopharmaceutical science, ion-exchange chromatography (IEC) is a well-known purification technique to separate the impurities such as host cell proteins from recombinant proteins. However, IEC is one of the limiting steps in the purification process of recombinant hepatitis B surface antigen (rH...

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Veröffentlicht in:	Protein expression and purification 2018-01, Vol.141, p.25-31
Hauptverfasser:	Mojarrad Moghanloo, Gol Mohammad, Khatami, Maryam, Javidanbardan, Amin, Hosseini, Seyed Nezamedin
Format:	Artikel
Sprache:	eng
Schlagworte:	Batch Cell Culture Techniques Buffers Chromatography, Ion Exchange - methods Chromatography, Ion Exchange - standards Conductivity Electric Conductivity Hepatitis B Surface Antigens - chemistry Hepatitis B Surface Antigens - genetics Hepatitis B Surface Antigens - isolation & purification Hepatitis B Surface Antigens - metabolism Optimization Pichia - genetics Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Recovery rHBsAg Scale up Weak anion exchange
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Beschreibung
Zusammenfassung:	In biopharmaceutical science, ion-exchange chromatography (IEC) is a well-known purification technique to separate the impurities such as host cell proteins from recombinant proteins. However, IEC is one of the limiting steps in the purification process of recombinant hepatitis B surface antigen (rHBsAg), due to its low recovery rate (95%.
ISSN:	1046-5928 1096-0279
DOI:	10.1016/j.pep.2017.08.011