Effects of anesthesia (tricaine methanesulfonate, MS222) on liver biotransformation in rainbow trout ( Oncorhynchus mykiss)
The effect of tricaine methanesulfonate (MS222) on rainbow trout liver biotransformation rates was investigated with a microsomal model; an in vitro preparation that can be employed with or without the use of an anaesthetic. Two experimental sets of rainbow trout microsomes were tested; one represen...
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Veröffentlicht in: | Aquatic toxicology 2003-07, Vol.64 (2), p.177-184 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The effect of tricaine methanesulfonate (MS222) on rainbow trout liver biotransformation rates was investigated with a microsomal model; an in vitro preparation that can be employed with or without the use of an anaesthetic. Two experimental sets of rainbow trout microsomes were tested; one representing in vivo or surgical tricaine exposures and the other representing in vitro tissue/organ collection tricaine exposures. Microsomal incubations were performed on these two experimental groups with phenol as substrate to assess the effects of tricaine on Phase I (ring-hydroxylation) and II (glucuronidation) liver biotransformation by monitoring production of hydroquinone (HQ), catechol (CAT), and phenylglucuronide (PG). The use of a 2-h 100 mg/l exposure of tricaine for surgical anesthesia with or without 24-h recovery did not significantly (
P≤0.05) affect rates of phenol (Phase I and II) biotransformation rates; nor, did the 5-min 300 mg/l tricaine exposure for isolated organ/tissue collection significantly (
P≤0.05) affect phenol (Phase I and II) biotransformation rates. There were also no significant statistical differences (
P≤0.05) in P450 protein levels, or 7-ethoxyresorufin-
O-deethylase (EROD) activity in these microsomal assays between any of the tricaine treated rainbow trout and controls. |
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ISSN: | 0166-445X 1879-1514 |
DOI: | 10.1016/S0166-445X(03)00051-1 |