Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe

A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex , [Ir(phq) (phen)]PF (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadr...

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Veröffentlicht in:Chemical science (Cambridge) 2015-04, Vol.6 (4), p.2166-2171
Hauptverfasser: Leung, Ka-Ho, He, Hong-Zhang, He, Bingyong, Zhong, Hai-Jing, Lin, Sheng, Wang, Yi-Tao, Ma, Dik-Lung, Leung, Chung-Hang
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Sprache:eng
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Zusammenfassung:A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex , [Ir(phq) (phen)]PF (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.
ISSN:2041-6520
2041-6539
DOI:10.1039/c4sc03319a