Peripheral Gamma Delta T cells secrete inflammatory cytokines in women with idiopathic recurrent pregnancy loss

•CD3+CD4−CD8− γδ T cells are the major subset of γδ T cells in RPL.•These cells are mainly involved in the production of IFNγ & IL17 in the circulation.•Proportion of TGFβ producing CD3+CD4−CD8− γδ T cells are less in RPL than in control.•Enhanced IFN-γ and IL-17 by γδ T cells contributes towards inflammation in RPL. Gamma delta (γδ) T cells are known to link innate and adaptive immunity. Decidual γδ T cells are known to provide immunotolerance by producing IL-10 and TGF-β. In recurrent pregnancy loss (RPL) females, the role of peripheral γδ T cells remain unstudied. To investigate the different phenotypes of γδ T cells in the peripheral blood of women with idiopathic RPL and their possible involvement in RPL condition. A total of 120 women were recruited for the study. Peripheral blood lymphocytes were isolated and they were stained with appropriate antibodies to determine the phenotype of γδ T cells and major cytokines produced by them in the blood using flow cytometry. We observed a significant decrease in the proportion of CD3+CD4−CD8−γδ T cells (p