Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by GeneXpert MTB/RIF
GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated s...
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| Veröffentlicht in: | Tuberculosis (Edinburgh, Scotland) Scotland), 2017-09, Vol.106, p.53-55 |
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| container_title | Tuberculosis (Edinburgh, Scotland) |
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| creator | Rindi, Laura Alì, Greta Fabiani, Barbara Fontanini, Gabriella Garzelli, Carlo |
| description | GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated successful protocol for processing paraffin-embedded tissue and assess the feasibility of the Xpert assay-based tuberculosis (TB) diagnosis on these specimens, thus proving the Xpert assay as a valuable TB diagnostic tool in supporting conventional histopathological methods. |
| doi_str_mv | 10.1016/j.tube.2017.06.005 |
| format | Article |
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| subjects | Antibiotics, Antitubercular - pharmacology Assaying Automation, Laboratory Bacteria Bacteriological Techniques Bioassays Biopsy Case-Control Studies Diagnostic software Diagnostic systems DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Drug Resistance, Bacterial - genetics Feasibility studies Humans Medical diagnosis Microbial Sensitivity Tests Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Paraffin Paraffin Embedding Paraffins Predictive Value of Tests Real-Time Polymerase Chain Reaction - methods Rifampin Rifampin - pharmacology Tuberculosis Tuberculosis, Lymph Node - diagnosis Tuberculosis, Lymph Node - genetics Tuberculosis, Lymph Node - pathology Tuberculosis, Pleural - diagnosis Tuberculosis, Pleural - genetics Tuberculosis, Pleural - pathology Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - genetics Tuberculosis, Pulmonary - pathology |
| title | Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by GeneXpert MTB/RIF |
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