Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by GeneXpert MTB/RIF

GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated s...

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Veröffentlicht in:	Tuberculosis (Edinburgh, Scotland) Scotland), 2017-09, Vol.106, p.53-55
Hauptverfasser:	Rindi, Laura, Alì, Greta, Fabiani, Barbara, Fontanini, Gabriella, Garzelli, Carlo
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotics, Antitubercular - pharmacology Assaying Automation, Laboratory Bacteria Bacteriological Techniques Bioassays Biopsy Case-Control Studies Diagnostic software Diagnostic systems DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Drug Resistance, Bacterial - genetics Feasibility studies Humans Medical diagnosis Microbial Sensitivity Tests Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Paraffin Paraffin Embedding Paraffins Predictive Value of Tests Real-Time Polymerase Chain Reaction - methods Rifampin Rifampin - pharmacology Tuberculosis Tuberculosis, Lymph Node - diagnosis Tuberculosis, Lymph Node - genetics Tuberculosis, Lymph Node - pathology Tuberculosis, Pleural - diagnosis Tuberculosis, Pleural - genetics Tuberculosis, Pleural - pathology Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - genetics Tuberculosis, Pulmonary - pathology
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Zusammenfassung:	GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated successful protocol for processing paraffin-embedded tissue and assess the feasibility of the Xpert assay-based tuberculosis (TB) diagnosis on these specimens, thus proving the Xpert assay as a valuable TB diagnostic tool in supporting conventional histopathological methods.
ISSN:	1472-9792 1873-281X
DOI:	10.1016/j.tube.2017.06.005