Proteomics Investigations into Serum Proteins Adsorbed by High‐Flux and Low‐Flux Dialysis Membranes

Hemodialysis is one of the most important therapies for patients with uremia, and the dialysis membrane is the predominant factor that impacts the efficiency of dialysis. Here, a protein adsorption on two different membranes is investigated to provide a basis for improving dialysis materials. Two cases treated with the Polyflux 14L low‐flux dialyzer and the Polyflux 140H high‐flux dialyzers during two continuous therapies are selected. Four used dialyzers from selected patients are infused with C12Im‐Cl to elute the adsorbed proteins. Then labeled digested proteins adsorb by Polyflux 140H and Polyflux 14L with 13CD2O and NaCNBD3 (light labeling, L) and CD2O and NaCNBH3 (heavy labeling, H), respectively. Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) is used to identify the proteins. According to the ratio (Light labeling/Heavy labeling), the eluted proteins are divided into three groups: significantly higher, significantly lower, and no significant differences with a ratio of >2, 2), 320 proteins are retained more by the Polyflux 14L membrane (ratio