Targeted Protein Detection Using an All-in-One Mass Spectrometry Cartridge

Targeted Protein Detection Using an All-in-One Mass Spectrometry Cartridge

We developed a simple 3D printed cartridge for mass spectrometry (MS) targeted detection of plasma proteins, including post-translational modifications (PTMs). The cartridge uses an integrated antibody enrichment column to preconcentrate the protein target as well as a novel built-in substrate to io...

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Veröffentlicht in:Journal of the American Chemical Society 2017-08, Vol.139 (32), p.10996-10999
Hauptverfasser: Zhang, Chengsen, Glaros, Trevor, Manicke, Nicholas E
Format: Artikel
Sprache:eng
Schlagworte:
Blood Proteins - analysis
Equipment Design
Humans
Mass Spectrometry - instrumentation
Printing, Three-Dimensional
Protein Processing, Post-Translational
Proteomics - instrumentation
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Zusammenfassung:We developed a simple 3D printed cartridge for mass spectrometry (MS) targeted detection of plasma proteins, including post-translational modifications (PTMs). The cartridge uses an integrated antibody enrichment column to preconcentrate the protein target as well as a novel built-in substrate to ionize the protein targets for MS detection. We show several examples of using this cartridge to perform rapid detection of clinically significant proteoforms from plasma samples.
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.7b05571