A simple protocol for isolation of fungal DNA

Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population st...

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Veröffentlicht in:	Biotechnology letters 2003-11, Vol.25 (22), p.1941-1944
Hauptverfasser:	TENDULKAR, Sachin R, GUPTA, Archna, CHATTOO, Bharat B
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Deoxyribonucleic acid DNA DNA, Fungal - chemistry DNA, Fungal - isolation & purification Dose-Response Relationship, Radiation Electromagnetic Fields Fundamental and applied biological sciences. Psychology Magnaporthe - chemistry Magnaporthe - genetics Magnaporthe - isolation & purification Magnaporthe - radiation effects Magnaporthe grisea Microchemistry - methods Microwaves Population structure Radiation Dosage
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Beschreibung
Zusammenfassung:	Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phytopathogen in different regions, and for a quick screening of M. grisea transformants.
ISSN:	0141-5492 1573-6776
DOI:	10.1023/B:BILE.0000003990.27624.04