Combined effects of ionizing-irradiation and different environments on Clostridium botulinum type E spores

We examined the combined effects of γ-radiation (24 °C) on spores of Clostridium botulinum-type Eklund strain suspended in different gas-saturated Na-phosphate buffer in absence or presence of protectors or sensitizers. Response surface methodology (RSM) was also used to ascertain the effects of rad...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:International journal of food microbiology 2003-12, Vol.89 (2), p.251-263
Hauptverfasser: Lim, Y.H, Hamdy, M.K, Toledo, R.T
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 263
container_issue 2
container_start_page 251
container_title International journal of food microbiology
container_volume 89
creator Lim, Y.H
Hamdy, M.K
Toledo, R.T
description We examined the combined effects of γ-radiation (24 °C) on spores of Clostridium botulinum-type Eklund strain suspended in different gas-saturated Na-phosphate buffer in absence or presence of protectors or sensitizers. Response surface methodology (RSM) was also used to ascertain the effects of radiation on the recovery of spores using a medium containing various levels of NaCl or Na-thioglycollate. The former (
doi_str_mv 10.1016/S0168-1605(03)00156-9
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19195378</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168160503001569</els_id><sourcerecordid>19195378</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-f3809f1411947e074b9cc6df4fbe6fee9cdf1bbb49f7743d8fc3b604c919ee9d3</originalsourceid><addsrcrecordid>eNqFkU1vFSEUhomxsdfqT9Cw0ehi9HBhPlg15qZakyZdqGvCwMGcZgauMNOk_npp741ddgMnh-eFkwfG3gj4JEB0n3_UZWhEB-0HkB8BRNs1-hnbiKHXjVQdPGeb_8gpe1nKDQC0UsILdipUt5VSiw272aV5pIieYwjolsJT4JQi_aX4u6GcrSe71Aa30XNPFcoYF47xlnKKc61rJPLdlMqSydM68zEt60SxVsvdHvkFL_uUsbxiJ8FOBV8f9zP26-vFz91lc3X97fvuy1Xj1Ha7NEEOoINQQmjVI_Rq1M51PqgwYhcQtfNBjOOodOh7Jf0QnBw7UE4LXU-9PGPvD_fuc_qzYlnMTMXhNNmIaS1GVLCV_VDB9gC6nErJGMw-02zznRFg7iWbB8nm3qABaR4kG11zb48PrOOM_jF1tFqBd0fAFmenkG10VB65tpUgoK_c-YHDquOWMJviCKNDT7n-hfGJnhjlH3rzm6A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19195378</pqid></control><display><type>article</type><title>Combined effects of ionizing-irradiation and different environments on Clostridium botulinum type E spores</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Lim, Y.H ; Hamdy, M.K ; Toledo, R.T</creator><creatorcontrib>Lim, Y.H ; Hamdy, M.K ; Toledo, R.T</creatorcontrib><description>We examined the combined effects of γ-radiation (24 °C) on spores of Clostridium botulinum-type Eklund strain suspended in different gas-saturated Na-phosphate buffer in absence or presence of protectors or sensitizers. Response surface methodology (RSM) was also used to ascertain the effects of radiation on the recovery of spores using a medium containing various levels of NaCl or Na-thioglycollate. The former (&lt;0.5%) decreased viable spore counts, but the latter (0.15%) did not. Irradiation inactivation of Eklund spores was most effective in air-saturated buffers compared to N 2O and N 2 gas. The Na 2–EDTA (0.01 M) was the most efficient radioprotector of spores due to its reactivity toward hydroxy radicals, followed by t-butanol (0.1 M) in NO 2 or N 2-saturated buffers, respectively. Catalase (10.0 mg ml −1) and dl-cysteine (0.1 mM) sensitized the spores during irradiated N 2O or N 2-saturated buffers, and NaCl (0.01 M) only sensitized spores in N 2 environment. Spores frozen at −75 °C for 30 days and thawed prior to use were more sensitive to radiation damage compared to freshly prepared spores. Glycerol (15%), in Na-phosphate buffer (pH 7.0, 0.06 M), protected Eklund spores and increased the number of spores from 10 6 to 10 11 colony forming unit (CFU) ml −1, and enhanced their radiosensitivities. Seven strains of C. botulinum type E were screened for plasmids and strain BL764 had two plasmids (15.8 and 46.8 mDa), BL4028 also had two (4.4 and 13.2 mDa), BL4850 contained only one (4.9 mDa), whereas EQA, BL211, Eklund, and Beluga had none. γ-Radiation (10 kGy, absorbed dose) cured the 15.8-mDa plasmid in strain BL764, but its absence yielded no changes in toxigenicity.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/S0168-1605(03)00156-9</identifier><identifier>PMID: 14623391</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; C. botulinum ; Clostridium botulinum ; Clostridium botulinum - drug effects ; Clostridium botulinum - physiology ; Clostridium botulinum - radiation effects ; Colony Count, Microbial ; Culture Media - chemistry ; Dose-Response Relationship, Drug ; Dose-Response Relationship, Radiation ; Food industries ; Food Irradiation ; Food microbiology ; Fundamental and applied biological sciences. Psychology ; Gamma Rays ; Hydrogen-Ion Concentration ; Plasmid curing ; Radioprotector ; Radiosensitizers ; Sodium Chloride - pharmacology ; Spores, Bacterial - drug effects ; Spores, Bacterial - radiation effects ; Thioglycolates - pharmacology ; γ-Irradiation</subject><ispartof>International journal of food microbiology, 2003-12, Vol.89 (2), p.251-263</ispartof><rights>2003 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-f3809f1411947e074b9cc6df4fbe6fee9cdf1bbb49f7743d8fc3b604c919ee9d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168160503001569$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=15530107$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14623391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lim, Y.H</creatorcontrib><creatorcontrib>Hamdy, M.K</creatorcontrib><creatorcontrib>Toledo, R.T</creatorcontrib><title>Combined effects of ionizing-irradiation and different environments on Clostridium botulinum type E spores</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>We examined the combined effects of γ-radiation (24 °C) on spores of Clostridium botulinum-type Eklund strain suspended in different gas-saturated Na-phosphate buffer in absence or presence of protectors or sensitizers. Response surface methodology (RSM) was also used to ascertain the effects of radiation on the recovery of spores using a medium containing various levels of NaCl or Na-thioglycollate. The former (&lt;0.5%) decreased viable spore counts, but the latter (0.15%) did not. Irradiation inactivation of Eklund spores was most effective in air-saturated buffers compared to N 2O and N 2 gas. The Na 2–EDTA (0.01 M) was the most efficient radioprotector of spores due to its reactivity toward hydroxy radicals, followed by t-butanol (0.1 M) in NO 2 or N 2-saturated buffers, respectively. Catalase (10.0 mg ml −1) and dl-cysteine (0.1 mM) sensitized the spores during irradiated N 2O or N 2-saturated buffers, and NaCl (0.01 M) only sensitized spores in N 2 environment. Spores frozen at −75 °C for 30 days and thawed prior to use were more sensitive to radiation damage compared to freshly prepared spores. Glycerol (15%), in Na-phosphate buffer (pH 7.0, 0.06 M), protected Eklund spores and increased the number of spores from 10 6 to 10 11 colony forming unit (CFU) ml −1, and enhanced their radiosensitivities. Seven strains of C. botulinum type E were screened for plasmids and strain BL764 had two plasmids (15.8 and 46.8 mDa), BL4028 also had two (4.4 and 13.2 mDa), BL4850 contained only one (4.9 mDa), whereas EQA, BL211, Eklund, and Beluga had none. γ-Radiation (10 kGy, absorbed dose) cured the 15.8-mDa plasmid in strain BL764, but its absence yielded no changes in toxigenicity.</description><subject>Biological and medical sciences</subject><subject>C. botulinum</subject><subject>Clostridium botulinum</subject><subject>Clostridium botulinum - drug effects</subject><subject>Clostridium botulinum - physiology</subject><subject>Clostridium botulinum - radiation effects</subject><subject>Colony Count, Microbial</subject><subject>Culture Media - chemistry</subject><subject>Dose-Response Relationship, Drug</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Food industries</subject><subject>Food Irradiation</subject><subject>Food microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gamma Rays</subject><subject>Hydrogen-Ion Concentration</subject><subject>Plasmid curing</subject><subject>Radioprotector</subject><subject>Radiosensitizers</subject><subject>Sodium Chloride - pharmacology</subject><subject>Spores, Bacterial - drug effects</subject><subject>Spores, Bacterial - radiation effects</subject><subject>Thioglycolates - pharmacology</subject><subject>γ-Irradiation</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vFSEUhomxsdfqT9Cw0ehi9HBhPlg15qZakyZdqGvCwMGcZgauMNOk_npp741ddgMnh-eFkwfG3gj4JEB0n3_UZWhEB-0HkB8BRNs1-hnbiKHXjVQdPGeb_8gpe1nKDQC0UsILdipUt5VSiw272aV5pIieYwjolsJT4JQi_aX4u6GcrSe71Aa30XNPFcoYF47xlnKKc61rJPLdlMqSydM68zEt60SxVsvdHvkFL_uUsbxiJ8FOBV8f9zP26-vFz91lc3X97fvuy1Xj1Ha7NEEOoINQQmjVI_Rq1M51PqgwYhcQtfNBjOOodOh7Jf0QnBw7UE4LXU-9PGPvD_fuc_qzYlnMTMXhNNmIaS1GVLCV_VDB9gC6nErJGMw-02zznRFg7iWbB8nm3qABaR4kG11zb48PrOOM_jF1tFqBd0fAFmenkG10VB65tpUgoK_c-YHDquOWMJviCKNDT7n-hfGJnhjlH3rzm6A</recordid><startdate>20031231</startdate><enddate>20031231</enddate><creator>Lim, Y.H</creator><creator>Hamdy, M.K</creator><creator>Toledo, R.T</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20031231</creationdate><title>Combined effects of ionizing-irradiation and different environments on Clostridium botulinum type E spores</title><author>Lim, Y.H ; Hamdy, M.K ; Toledo, R.T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-f3809f1411947e074b9cc6df4fbe6fee9cdf1bbb49f7743d8fc3b604c919ee9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>C. botulinum</topic><topic>Clostridium botulinum</topic><topic>Clostridium botulinum - drug effects</topic><topic>Clostridium botulinum - physiology</topic><topic>Clostridium botulinum - radiation effects</topic><topic>Colony Count, Microbial</topic><topic>Culture Media - chemistry</topic><topic>Dose-Response Relationship, Drug</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Food industries</topic><topic>Food Irradiation</topic><topic>Food microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gamma Rays</topic><topic>Hydrogen-Ion Concentration</topic><topic>Plasmid curing</topic><topic>Radioprotector</topic><topic>Radiosensitizers</topic><topic>Sodium Chloride - pharmacology</topic><topic>Spores, Bacterial - drug effects</topic><topic>Spores, Bacterial - radiation effects</topic><topic>Thioglycolates - pharmacology</topic><topic>γ-Irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lim, Y.H</creatorcontrib><creatorcontrib>Hamdy, M.K</creatorcontrib><creatorcontrib>Toledo, R.T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lim, Y.H</au><au>Hamdy, M.K</au><au>Toledo, R.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined effects of ionizing-irradiation and different environments on Clostridium botulinum type E spores</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2003-12-31</date><risdate>2003</risdate><volume>89</volume><issue>2</issue><spage>251</spage><epage>263</epage><pages>251-263</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>We examined the combined effects of γ-radiation (24 °C) on spores of Clostridium botulinum-type Eklund strain suspended in different gas-saturated Na-phosphate buffer in absence or presence of protectors or sensitizers. Response surface methodology (RSM) was also used to ascertain the effects of radiation on the recovery of spores using a medium containing various levels of NaCl or Na-thioglycollate. The former (&lt;0.5%) decreased viable spore counts, but the latter (0.15%) did not. Irradiation inactivation of Eklund spores was most effective in air-saturated buffers compared to N 2O and N 2 gas. The Na 2–EDTA (0.01 M) was the most efficient radioprotector of spores due to its reactivity toward hydroxy radicals, followed by t-butanol (0.1 M) in NO 2 or N 2-saturated buffers, respectively. Catalase (10.0 mg ml −1) and dl-cysteine (0.1 mM) sensitized the spores during irradiated N 2O or N 2-saturated buffers, and NaCl (0.01 M) only sensitized spores in N 2 environment. Spores frozen at −75 °C for 30 days and thawed prior to use were more sensitive to radiation damage compared to freshly prepared spores. Glycerol (15%), in Na-phosphate buffer (pH 7.0, 0.06 M), protected Eklund spores and increased the number of spores from 10 6 to 10 11 colony forming unit (CFU) ml −1, and enhanced their radiosensitivities. Seven strains of C. botulinum type E were screened for plasmids and strain BL764 had two plasmids (15.8 and 46.8 mDa), BL4028 also had two (4.4 and 13.2 mDa), BL4850 contained only one (4.9 mDa), whereas EQA, BL211, Eklund, and Beluga had none. γ-Radiation (10 kGy, absorbed dose) cured the 15.8-mDa plasmid in strain BL764, but its absence yielded no changes in toxigenicity.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>14623391</pmid><doi>10.1016/S0168-1605(03)00156-9</doi><tpages>13</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0168-1605
ispartof International journal of food microbiology, 2003-12, Vol.89 (2), p.251-263
issn 0168-1605
1879-3460
language eng
recordid cdi_proquest_miscellaneous_19195378
source MEDLINE; Elsevier ScienceDirect Journals
subjects Biological and medical sciences
C. botulinum
Clostridium botulinum
Clostridium botulinum - drug effects
Clostridium botulinum - physiology
Clostridium botulinum - radiation effects
Colony Count, Microbial
Culture Media - chemistry
Dose-Response Relationship, Drug
Dose-Response Relationship, Radiation
Food industries
Food Irradiation
Food microbiology
Fundamental and applied biological sciences. Psychology
Gamma Rays
Hydrogen-Ion Concentration
Plasmid curing
Radioprotector
Radiosensitizers
Sodium Chloride - pharmacology
Spores, Bacterial - drug effects
Spores, Bacterial - radiation effects
Thioglycolates - pharmacology
γ-Irradiation
title Combined effects of ionizing-irradiation and different environments on Clostridium botulinum type E spores
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T04%3A58%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Combined%20effects%20of%20ionizing-irradiation%20and%20different%20environments%20on%20Clostridium%20botulinum%20type%20E%20spores&rft.jtitle=International%20journal%20of%20food%20microbiology&rft.au=Lim,%20Y.H&rft.date=2003-12-31&rft.volume=89&rft.issue=2&rft.spage=251&rft.epage=263&rft.pages=251-263&rft.issn=0168-1605&rft.eissn=1879-3460&rft.coden=IJFMDD&rft_id=info:doi/10.1016/S0168-1605(03)00156-9&rft_dat=%3Cproquest_cross%3E19195378%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19195378&rft_id=info:pmid/14623391&rft_els_id=S0168160503001569&rfr_iscdi=true