An alkylating immobilization linker for immunochemical epigenetic assessment

A bifunctional linker molecule containing nitrogen mustard and a cyclic disulfide group has been developed for the covalent immobilization of intact DNA, which allows quantitative analysis of epigenomic modification in immobilized DNA using SPR-based immune sensing.

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2017, Vol.53 (59), p.8308-8311
Hauptverfasser:	Kurinomaru, Takaaki, Kojima, Naoshi, Kurita, Ryoji
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkylation Cross-Linking Reagents - chemical synthesis Cross-Linking Reagents - chemistry Disulfides - chemistry DNA - chemistry DNA - genetics Epigenesis, Genetic Immunochemistry Mechlorethamine - chemistry Molecular Structure Surface Plasmon Resonance
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A bifunctional linker molecule containing nitrogen mustard and a cyclic disulfide group has been developed for the covalent immobilization of intact DNA, which allows quantitative analysis of epigenomic modification in immobilized DNA using SPR-based immune sensing.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c7cc02883k