Capillary electrophoresis coupled with chloroform‐acetonitrile extraction for rapid and highly selective determination of cysteine and homocysteine levels in human blood plasma and urine

A rapid and selective method has been developed for highly sensitive determination of total cysteine and homocysteine levels in human blood plasma and urine by capillary electrophoresis (CE) coupled with liquid–liquid extraction. Analytes were first derivatized with 1,1′‐thiocarbonyldiimidazole and...

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Veröffentlicht in:Electrophoresis 2017-10, Vol.38 (20), p.2646-2653
Hauptverfasser: Ivanov, Alexander Vladimirovich, Bulgakova, Polina Olegovna, Virus, Edward Danielevich, Kruglova, Maria Petrovna, Alexandrin, Valery Vasil'evich, Gadieva, Viktoriya Aleksandrovna, Luzyanin, Boris Petrovich, Kushlinskii, Nikolai Evgen'evich, Fedoseev, Anatolij Nikolaevich, Kubatiev, Aslan Amirkhanovich
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Sprache:eng
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Zusammenfassung:A rapid and selective method has been developed for highly sensitive determination of total cysteine and homocysteine levels in human blood plasma and urine by capillary electrophoresis (CE) coupled with liquid–liquid extraction. Analytes were first derivatized with 1,1′‐thiocarbonyldiimidazole and then samples were purified by chloroform–ACN extraction. Electrophoretic separation was performed using 0.1 M phosphate with 30 mM triethanolamine, pH 2, containing 25 μM CTAB, 2.5 μM SDS, and 2.5% polyethylene glycol 600. Samples were injected into the capillary (with total length 32 cm and 50 μm id) at 2250 mbar*s and subsequent injection was performed for 30 s with 0.5 M KОН. The total analysis time was less than 9 min, accuracy was 98%, and precision was
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201700133