Bosea minatitlanensis sp. nov., a strictly aerobic bacterium isolated from an anaerobic digester

1 Département de Biochimie-Microbiologie, Unité de Formation et de Recherches en Sciences de la Vie et de la Terre, Université de Ouagadougou, 03 BP 7021, Ouagadougou 03, Burkina Faso 2 Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Avenida Michoacán y la Purisima s/n, Col. Vicentina, 09340 México DF, Mexico 3 Institut de Recherche pour le Développement (IRD), Cicerón 609, Col. Los Morales, 11530 México DF, Mexico 4 Laboratoire de Microbiologie IRD, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163, avenue de Luminy, 13288 Marseille cedex 9, France Correspondence Aboubakar S. Ouattara ouattabs{at}univ-ouaga.bf A strictly aerobic, mesophilic bacterium, strain AMX 51 T , was isolated from anaerobic digester sludge. Cells were Gram-negative, motile, non-sporulating, straight to curved rods with one polar flagellum. The isolate had phenotypic traits of the genus Bosea , including cellular fatty acid and substrate utilization profiles. Physiological characteristics and antibiotic susceptibility were determined. Phylogenetic analysis revealed that strain AMX 51 T was a member of the - Proteobacteria , most closely related to Bosea thiooxidans DSM 9653 T (similarity of 98·88 %). Methylobacterium organophilum JCM 2833 T , Methylobacterium mesophilicum JCM 2829 T , Afipia clevelandensis DSM 7315 T , Afipia felis DSM 7326 T , Afipia broomeae DSM 7327 T , Blastobacter denitrificans LMG 8443 T and Bradyrhizobium japonicum DSM 30131 T showed significant 16S rRNA gene sequence similarities to strain AMX 51 T . The DNA G+C composition of strain AMX 51 T was 68·5 mol%. DNA–DNA hybridization analysis revealed 44·2 and 15·1 % relatedness between strain AMX 51 T and the respective type strains of Bosea thiooxidans and A. felis . Overall results suggest that strain AMX 51 T (=DSM 13099 T =ATCC 700918 T =CIP 106457 T ) represents a novel species of the genus Bosea ; the name Bosea minatitlanensis sp. nov. is proposed. Abbreviations: CFA, cellular fatty acid; UASB reactor, upflow anaerobic sludge blanket reactor The GenBank accession number for the 16S rRNA gene sequence of strain AMX 51 T is AF273081 .