A plate-based histo-blood group antigen binding assay for evaluation of human norovirus receptor binding ability

Human norovirus is a leading cause of gastroenteritis worldwide. Although two in vitro cultivation methods have been reported, they cannot provide mechanistic insights into viral inactivation. Receptor-binding assays supplement these assays and give insight into capsid integrity. We present a stream...

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Veröffentlicht in:	Analytical biochemistry 2017-09, Vol.533, p.56-59
Hauptverfasser:	Moore, Matthew D., Jaykus, Lee-Ann
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence - genetics Blood Group Antigens - immunology Blood Group Antigens - isolation & purification Capsid functionality Capsid Proteins - blood Disinfection Gastroenteritis - diagnosis Gastroenteritis - virology Genotype Histo-blood group antigens Humans Norovirus Norovirus - genetics Norovirus - immunology Norovirus - isolation & purification Norovirus - pathogenicity Protein Binding Receptor binding assay
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Zusammenfassung:	Human norovirus is a leading cause of gastroenteritis worldwide. Although two in vitro cultivation methods have been reported, they cannot provide mechanistic insights into viral inactivation. Receptor-binding assays supplement these assays and give insight into capsid integrity. We present a streamlined version of a receptor-binding assay with minimal time-to-result while maintaining accuracy and high throughput. We validate assay performance for physical and chemical inactivation treatments of a norovirus GII.4 capsid. The assay produces a high positive/negative ratio (25.3 ± 4.9) in
ISSN:	0003-2697 1096-0309
DOI:	10.1016/j.ab.2017.06.012