Micro/extended-nano sampling interface from a living single cell
Single-cell analysis is of increasing importance in many fields, but is challenging due to the ultra-small volumes (picoliters) of single cells. Indeed, analysis of a specific analyte might require the analysis of a single molecule or several molecules. Analytical processes usually include sampling,...
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| Veröffentlicht in: | Analyst (London) 2017-05, Vol.142 (10), p.1689-1696 |
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| creator | Lin, L Mawatari, K Morikawa, K Pihosh, Y Yoshizaki, A Kitamori, T |
| description | Single-cell analysis is of increasing importance in many fields, but is challenging due to the ultra-small volumes (picoliters) of single cells. Indeed, analysis of a specific analyte might require the analysis of a single molecule or several molecules. Analytical processes usually include sampling, chemical processing, and detection. Although several papers have reported chemical processing and detection methods for single cells, a sampling method compatible with maintaining the viability of a single cell during sampling has yet to be developed. Here, we propose a femtoliter sampling method from a living single cell using micro/nanofluidic device technology. The sampling of 39 fL of cytoplasm from a single human aortic endothelial cell was demonstrated and its viability after sampling was confirmed. |
| doi_str_mv | 10.1039/c7an00220c |
| format | Article |
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Indeed, analysis of a specific analyte might require the analysis of a single molecule or several molecules. Analytical processes usually include sampling, chemical processing, and detection. Although several papers have reported chemical processing and detection methods for single cells, a sampling method compatible with maintaining the viability of a single cell during sampling has yet to be developed. Here, we propose a femtoliter sampling method from a living single cell using micro/nanofluidic device technology. The sampling of 39 fL of cytoplasm from a single human aortic endothelial cell was demonstrated and its viability after sampling was confirmed.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c7an00220c</identifier><identifier>PMID: 28393168</identifier><language>eng</language><publisher>England</publisher><subject>Aorta - cytology ; Cells, Cultured ; Compatibility ; Cytoplasm - chemistry ; Devices ; Endothelial cells ; Endothelial Cells - cytology ; Humans ; Mathematical analysis ; Microfluidic Analytical Techniques ; Nanostructure ; Nanotechnology ; Sampling ; Sampling methods ; Single-Cell Analysis ; Viability</subject><ispartof>Analyst (London), 2017-05, Vol.142 (10), p.1689-1696</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-e74baeca1b9ae05f4b01a1006d12769cc2effd49aa849231ecc438ae217958cc3</citedby><cites>FETCH-LOGICAL-c386t-e74baeca1b9ae05f4b01a1006d12769cc2effd49aa849231ecc438ae217958cc3</cites><orcidid>0000-0001-7232-5531 ; 0000-0002-9163-9373 ; 0000-0001-9265-8237 ; 0000-0002-0117-7065 ; 0000-0003-2969-6550 ; 0000-0002-8194-9140</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2831,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28393168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, L</creatorcontrib><creatorcontrib>Mawatari, K</creatorcontrib><creatorcontrib>Morikawa, K</creatorcontrib><creatorcontrib>Pihosh, Y</creatorcontrib><creatorcontrib>Yoshizaki, A</creatorcontrib><creatorcontrib>Kitamori, T</creatorcontrib><title>Micro/extended-nano sampling interface from a living single cell</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>Single-cell analysis is of increasing importance in many fields, but is challenging due to the ultra-small volumes (picoliters) of single cells. 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| fulltext | fulltext |
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| ispartof | Analyst (London), 2017-05, Vol.142 (10), p.1689-1696 |
| issn | 0003-2654 1364-5528 |
| language | eng |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Aorta - cytology Cells, Cultured Compatibility Cytoplasm - chemistry Devices Endothelial cells Endothelial Cells - cytology Humans Mathematical analysis Microfluidic Analytical Techniques Nanostructure Nanotechnology Sampling Sampling methods Single-Cell Analysis Viability |
| title | Micro/extended-nano sampling interface from a living single cell |
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