A review of high performance liquid chromatographic-mass spectrometric urinary methods for anticancer drug exposure of health care workers

•Review of HPLC–MS/MS methods for quantitation of anticancer drugs in urine.•Includes drugs, isomers, drug metabolites and simultaneous multi-drug determinations.•Tabular summaries of method sample preparation, LC parameters, and MS detection modes.•Presents comparative overview of methods, their development, and validation protocols.•Discussion of future trends including UHPLC–MS/MS and simultaneous multi-drug methods. This review describes published high performance liquid chromatography/mass spectrometry (HPLC–MS) methods for the determination of anticancer drugs in human urine as non-invasive tool for monitoring of health care worker exposure to antineoplastic and cytotoxic drugs. HPLC–MS is a sensitive and specific method for analysis of anticancer drugs and their metabolites in biological fluids. In this review, a tabular summary and overview of published HPLC–MS methods are presented, as well as future trends and limitations in this area of research.