Coordinated Expression of acrAB-tolC and Eight Other Functional Efflux Pumps Through Activating ramA and marA in Salmonella enterica serovar Typhimurium
The aim of this study was to determine the expression of eight other functional transporter genes upon acrAB inactivation and also the expression of acrAB when the function of eight other transporters are impaired in Salmonella enterica . We used single- or multigene deletion mutants ( i.e. , Δ acrA...
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Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2018-03, Vol.24 (2), p.12-125 |
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Zusammenfassung: | The aim of this study was to determine the expression of eight other functional transporter genes upon
acrAB
inactivation and also the expression of
acrAB
when the function of eight other transporters are impaired in
Salmonella enterica
. We used single- or multigene deletion mutants (
i.e.
, Δ
acrA
, Δ
acrB
, Δ
tolC
, Δ
acrAB
, Δ
acrEF
, Δ
acrD
, Δ
mdsABC
, Δ
mdtABC
, Δ
emrAB
, Δ
macAB
, Δ
mdfA
, Δ
mdtK
, Δ
acrABramA
, Δ
acrABmarA
, and Δ
acrABsoxS
) and real time (RT)-PCR to quantify the expression of different pump and regulator genes; infection ability was characterized by adhesion and invasion assays. The expression of
acrAB
operon was increased upon
acrB
inactivation. Single deletion of
acrA
or
tolC
also increased expression of
acrB
. The deletion of
acrAB
increased expression of eight other functional efflux pumps genes and vice versa, in which increased expression of
ramA
and
marA
was also detected. Mutants containing single deletions of functional pump genes were attenuated in cells. In conclusion, there is a feedback mechanism that coordinates regulation of AcrAB-TolC and eight other functional efflux pumps through the global transcriptional regulators
ramA
and
marA
in
S. enterica
serovar Typhimurium. |
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ISSN: | 1076-6294 1931-8448 |
DOI: | 10.1089/mdr.2017.0086 |