The effect of antimicrobial photodynamic therapy on the expression of novel methicillin resistance markers determined using cDNA-AFLP approach in Staphylococcus aureus

Highlights • cDNA-AFLP is suitable approach for identification of new target genes implicated in antimicrobial resistance. • Serine hydrolase family protein (Shfp) encoding gene was up-regulated in methicillin resistant Staphylococcus aureus (MRSA). • The Shfp encoding genes might be implicated in methicillin resistance in MRSA. • A conserved hypothetical protein (Chp) encoding gene that was up-regulated in MRSA might be implicated in methicillin resistance in MRSA. • sPDT would lead to reduction in resistance to methicillin in MRSA by suppressing the expression of the Shfp and Chp encoding genes.