The Medicago truncatula R2R3-MYB transcription factor gene MtMYBS1 enhances salinity tolerance when constitutively expressed in Arabidopsis thaliana

MYB-type proteins are known to participate in many stress responses, although their role in legumes is still less clear. Here, the isolation and characterization of MtMYBS1, an R2R3 MYB gene isolated from the model legume Medicago truncatula, is described. MtMYBS1 transcription was inducible by NaCl, polyethylene glycol or abscisic acid (ABA). When tested in yeast, its product was shown to have transactivational activity. The constitutive expression of MtMYBS1 in Arabidopsis thaliana seedlings mitigated the restriction on root growth imposed by either salinity or osmotic stress and raised their sensitivity to ABA. It also resulted in the plants being able to overcome several growth constraints and promoted activity in both the ABA-dependent and -independent stress-responsive pathways. In particular, it enhanced the transcription of P5CS, a gene which encodes a component of proline synthesis. MtMYBS1 may prove to be a useful gene for manipulating the salinity tolerance of legumes. •MtMYBS1 transcription was inducible by NaCl, polyethylene glycol or ABA.•MtMYBS1 enhances the salt tolerant ability of Arabidopsis thaliana by regulating the transcription of P5CS.