DOSY Analysis of Micromolar Analytes: Resolving Dilute Mixtures by SABRE Hyperpolarization

DOSY is an NMR spectroscopy technique that resolves resonances according to the analytes’ diffusion coefficients. It has found use in correlating NMR signals and estimating the number of components in mixtures. Applications of DOSY in dilute mixtures are, however, held back by excessively long measurement times. We demonstrate herein, how the enhanced NMR sensitivity provided by SABRE hyperpolarization allows DOSY analysis of low‐micromolar mixtures, thus reducing the concentration requirements by at least 100‐fold. How low can DOSY go? DOSY analysis of a low‐micromolar mixture is achieved by combining DOSY with nuclear spin hyperpolarization. SABRE hyperpolarization enhances NMR signals by two orders of magnitude, resolving six dilute analytes in a 35 min SABRE‐DOSY experiment.