Iron chelators inhibit the heme-degradation reaction by HutZ from Vibrio cholerae

Iron chelators inhibit the heme-degradation reaction by HutZ from Vibrio cholerae

HutZ is a heme-degrading enzyme. We found that the heme-degradation reaction by HutZ is inhibited by the iron chelators. Kinetic analysis of each heme-degradation step suggests that water molecules hydrogen bonded to Thr27 are involved in proton transfer to Fe(iii)-OO , and that this step is inhibit...

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Veröffentlicht in:Dalton transactions : an international journal of inorganic chemistry 2017, Vol.46 (16), p.5147-5150
Hauptverfasser: Dojun, Nobuhiko, Sekine, Yukari, Ishimori, Koichiro, Uchida, Takeshi
Format: Artikel
Sprache:eng
Schlagworte:
Chemical bonds
Enzymes
Hydrogen
Inhibition
Iron
Reaction kinetics
Vibrio
Vibrio cholerae
Water chemistry
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Zusammenfassung:HutZ is a heme-degrading enzyme. We found that the heme-degradation reaction by HutZ is inhibited by the iron chelators. Kinetic analysis of each heme-degradation step suggests that water molecules hydrogen bonded to Thr27 are involved in proton transfer to Fe(iii)-OO , and that this step is inhibited by iron chelators.
ISSN:1477-9226
1477-9234
DOI:10.1039/c7dt00121e