Site-specific antibody modification and immobilization on a microfluidic chip to promote the capture of circulating tumor cells and microemboli

We design and synthesize EpCAM antibodies with Fc-domain site-specific linkers that allow preferential alignment when coated on microfluidic devices for capturing circulating tumor cells (CTCs) from colorectal cancer patients. The aligned coating is shown to increase the capture efficiency of CTCs a...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2017, Vol.53 (29), p.4152-4155
Hauptverfasser:	Lai, Chian-Hui, Choon Lim, Syer, Wu, Liang-Chun, Wang, Chien-Fang, Tsai, Wen-Sy, Wu, Han-Chung, Chang, Ying-Chih
Format:	Artikel
Sprache:	eng
Schlagworte:	Alignment Antibodies Antibodies, Immobilized - chemistry Antibodies, Immobilized - immunology Cell Separation - methods Coating Colorectal Neoplasms - immunology Colorectal Neoplasms - pathology Devices Efficiency Enzyme-Linked Immunosorbent Assay Humans Intracranial Embolism - immunology Intracranial Embolism - pathology Microfluidic Analytical Techniques Microfluidics Molecular Conformation Neoplastic Cells, Circulating - immunology Neoplastic Cells, Circulating - pathology Synthesis Tumors
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Beschreibung
Zusammenfassung:	We design and synthesize EpCAM antibodies with Fc-domain site-specific linkers that allow preferential alignment when coated on microfluidic devices for capturing circulating tumor cells (CTCs) from colorectal cancer patients. The aligned coating is shown to increase the capture efficiency of CTCs and microemboli by 1.6 and 3.0-fold, respectively (both P < 0.05).
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c7cc00247e