Developmental and internal validation of a novel 13 loci STR multiplex method for Cannabis sativa DNA profiling

Developmental and internal validation of a novel 13 loci STR multiplex method for Cannabis sativa DNA profiling

Highlights • A novel 13 loci cannabis STR multiplex method was developed and validated. • Six new cannabis STR tetranucleotide markers were included. • A sequenced allelic ladder was designed to genotype 101 C. sativa samples. • The sensitivity is 0.13 ng DNA and the power of discrimination is 1 in...

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Veröffentlicht in:Legal medicine (Tokyo, Japan) Japan), 2017-05, Vol.26, p.33-40
Hauptverfasser: Houston, Rachel, Birck, Matthew, Hughes-Stamm, Sheree, Gangitano, David
Format: Artikel
Sprache:eng
Schlagworte:
Cannabis - genetics
Cannabis sativa
DNA typing
Forensic Genetics
Forensic plant science
Gene Frequency
Humans
Internal Medicine
Microsatellite Repeats
Reference population
Short tandem repeats
Species Specificity
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Zusammenfassung:Highlights • A novel 13 loci cannabis STR multiplex method was developed and validated. • Six new cannabis STR tetranucleotide markers were included. • A sequenced allelic ladder was designed to genotype 101 C. sativa samples. • The sensitivity is 0.13 ng DNA and the power of discrimination is 1 in 55 million. • Well-balanced heterozygous peaks with minimal artifacts and stutter were observed.
ISSN:1344-6223
1873-4162
DOI:10.1016/j.legalmed.2017.03.001