Focused microwave irradiation-assisted immunohistochemistry to study effects of ketamine on phospho-ERK expression in the mouse brain

Highlights • Established a head-focused microwave irradiation-assisted immunohistochemistry assay to detect phosphorylated proteins in the brain. • Single dose of Ketamine and RO 25-6981 produced a robust, dose- and time-dependent increase in pERK immunoreactive neurons. • Increase in pERK observed...

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Veröffentlicht in:	Brain research 2017-09, Vol.1670, p.86-95
Hauptverfasser:	Fernandes, Alda, Li, Yu-Wen
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antidepressant Antidepressive Agents - pharmacology Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism Cyclic AMP Response Element-Binding Protein - metabolism Depression - drug therapy Dorsal Raphe Nucleus - metabolism Extracellular Signal-Regulated MAP Kinases - metabolism Focused microwave irradiation Immunohistochemistry - methods Immunoreactivity Ketamine Ketamine - pharmacology Male Mice Microwaves Mitogen-Activated Protein Kinase 3 - metabolism Neurology NR2B Phenols - pharmacology Phospho-ERK Phosphoprotein Phosphorylation - drug effects Piperidines - pharmacology Prefrontal Cortex - physiology Receptors, AMPA - metabolism Reproducibility of Results Signal Transduction - drug effects
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Zusammenfassung:	Highlights • Established a head-focused microwave irradiation-assisted immunohistochemistry assay to detect phosphorylated proteins in the brain. • Single dose of Ketamine and RO 25-6981 produced a robust, dose- and time-dependent increase in pERK immunoreactive neurons. • Increase in pERK observed in prilimbic region of medial prefrontal cortex, central amygdala, dorsal raphe nucleus and locus coeruleus.
ISSN:	0006-8993 1872-6240
DOI:	10.1016/j.brainres.2017.05.008