Effect of Nerve Growth Factor on Neural Differentiation of Mouse Embryonic Stem Cells

The cholinergic, GABAergic, and catecholaminergic neurons derived from mouse embryonic stem cells in a culture medium supplemented with recombinant NGF were phenotyped and scored. NGF stimulated generation of neurons, but not neuronal progenitors from embryonic stem cells and affected the proportion...

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2017-03, Vol.162 (5), p.679-683
Hauptverfasser:	Antonov, S. A., Manuilova, E. S., Dolotov, O. V., Kobylyansky, A. G., Safina, D. R., Grivennikov, I. A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biomedical and Life Sciences Biomedicine Cell Biology Cell culture Cell differentiation Cell Proliferation Cells, Cultured Embryo cells Embryonic stem cells Embryos Experimental Biology Fibroblast Growth Factor 2 - physiology GABA Humans Internal Medicine Laboratory Medicine Mice Mouse Embryonic Stem Cells - physiology Nerve growth factor Nerve Growth Factor - pharmacology Nerve Growth Factor - physiology Neural stem cells Neurogenesis Neurons Pathology Physiological aspects Stem cell transplantation Stem cells Toxicity testing γ-Aminobutyric acid
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Zusammenfassung:	The cholinergic, GABAergic, and catecholaminergic neurons derived from mouse embryonic stem cells in a culture medium supplemented with recombinant NGF were phenotyped and scored. NGF stimulated generation of neurons, but not neuronal progenitors from embryonic stem cells and affected the proportion of specific types of neurons in cultures of differentiating embryonic stem cells. These findings open vista to employ NGF for generation of specific neuron types from embryonic stem cells for fundamental and toxicological studies.
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-017-3686-1