Prevalence of tdh and trh genes of vibrio parahaemolyticus in shrimp (Litopenaeus vannamei) from Indramayu, West Java

This research aimed to know the prevalence of pathogenic Vibrio parahaemolyticus in shrimp (Litopenaeus vannamei) from traditional and intensive shrimp ponds based on their possessionof tdh and trh genes. Bacterial isolation and phenotypic characterization was carried out using FDA BAM (2004), and confirmation based on virulent factor gene (tdh and trh genes) was conducted by Polymerase Chain Reaction (PCR). The result showed that 16/32 (50%) and 6/32 (18.8%) shrimp samples from traditional and intensive ponds contained Vibrio parahaemolyticus, respectively. Pathogenic Vibrio parahaemolyticus due to their possession of tdh gene isolated from traditional and intensive ponds were 81percent (13/16) and 50% (3/6), respectively. When trh gene was used as the basic for classification, pathogenic Vibrio parahaemolyticus obtained from traditional and intensive ponds were reaching up 15/16 (93.8%) and 4/6 (66.7%) respectively. Out of the 22 Vibrio parahaemolyticus isolates, 16 (72.7%) were pathogenic based on the possession of gene encoding for tdh and 19 (86.4%) can be classified as pathogen based on the trh gene. As many as 14 out of 22 (63.6%) Vibrio parahaemolyticus isolates owned both tdh and trh genes.Original Abstract: Penelitian ini bertujuan untuk mengetahui prevalensi Vibrio parahaemolyticus patogenik pada udang vaname yang berasal dari tambak tradisional dan intensif berdasarkan keberadaan gen tdh dan trh. Isolasi dan konfirmasi Vibrio parahaemolyticus mengacu pada BAM (2004), yang dilanjutkan dengan konfirmasi gen tdh dan trh Vibrio parahaemolyticus menggunakan metode PCR. Hasil identifikasi menunjukkan bahwa sebanyak 16/32 (50%) dan 6/32 (18,8%) udang dari tambak tradisional dan intensif positif Vibrio parahaemolyticus. Berdasarkan gen tdh, ditemukan Vibrio parahaemolyticus patogenik pada udang tambak tradisional dan intensif berturut-turut adalah 81% (13/16) dan 50% (3/6). Sementara itu, jika didasarkan pada gen trh, Vibrio parahaemolyticus patogenik pada udang tambak tradisional dan intensif berturut-turut adalah 15/16 (93,8%) dan 4/6 (66,7%). Secara keseluruhan prevalensi udang vaname yang positif gen tdh adalah sebesar 72,2% (16/22) gen trh sebesar 86,4% (19/22) dan yang memiliki kedua gen adalah sebanyak 63,6% (14/22).