Production of d-lactate using a pyruvate-producing Escherichia coli strain

To generate an organism capable of producing d-lactate, NAD + -dependent d-lactate dehydrogenase was expressed in our pyruvate-producing strain, Escherichia coli strain LAFCPCPt-accBC-aceE. After determining the optimal culture conditions for d-lactate production, 18.4 mM d-lactate was produced from...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2017-07, Vol.81 (7), p.1452-1455
Hauptverfasser:	Akita, Hironaga, Nakashima, Nobutaka, Hoshino, Tamotsu
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetyl-CoA Carboxylase - genetics Acetyl-CoA Carboxylase - metabolism Batch Cell Culture Techniques Biomass corn steep liquor Culture Media - chemistry Culture Media - metabolism d-lactate Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Fatty Acid Synthase, Type II - genetics Fatty Acid Synthase, Type II - metabolism Fermentation Gene Expression Regulation, Bacterial Lactate Dehydrogenases - genetics Lactate Dehydrogenases - metabolism Lactic Acid - biosynthesis Metabolic Engineering Pyruvic Acid - metabolism
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Zusammenfassung:	To generate an organism capable of producing d-lactate, NAD + -dependent d-lactate dehydrogenase was expressed in our pyruvate-producing strain, Escherichia coli strain LAFCPCPt-accBC-aceE. After determining the optimal culture conditions for d-lactate production, 18.4 mM d-lactate was produced from biomass-based medium without supplemental mineral or nitrogen sources. Our results show that d-lactate can be produced in simple batch fermentation processes.
ISSN:	0916-8451 1347-6947
DOI:	10.1080/09168451.2017.1318697