Assessment of HTLV-1 proviral load, LAT, BIM, c-FOS and RAD51 gene expression in adult T cell leukemia/lymphoma

Adult T cell leukemia/lymphoma (ATLL) is a life-threatening malignancy of HTLV-1 infected Th lymphocytes. In the present study host–virus interactions were investigated by assessment of HTLV-1 proviral load (PVL) and host gene expression. A cross-sectional study was carried out on 18 ATLL, 10 HAM/TS...

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Veröffentlicht in:	Medical microbiology and immunology 2017-08, Vol.206 (4), p.327-335
Hauptverfasser:	Ramezani, Samaneh, Shirdel, Abbas, Rafatpanah, Houshang, Akbarin, Mohammad Mehdi, Tarokhian, Hanieh, Rahimi, Hossein, Bari, Alireza, Jahantigh, Hamid Reza, Rezaee, Seyed Abdolrahim
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptor Proteins, Signal Transducing - analysis Adult Adult T cell leukemia Attitude control Bcl-2-Like Protein 11 - analysis Biomedical and Life Sciences Biomedicine c-Fos protein Cross-Sectional Studies Deoxyribonucleic acid DNA DNA damage DNA repair Female Gene expression Gene Expression Profiling Genetic transformation Growth factors Human T-lymphotropic virus 1 - isolation & purification Humans Immunology Leukemia Leukemia-Lymphoma, Adult T-Cell - pathology Leukemia-Lymphoma, Adult T-Cell - virology Leukocytes (mononuclear) Leukocytes, Mononuclear - pathology Leukocytes, Mononuclear - virology Lymphocytes Lymphocytes T Lymphoma Male Malignancy Medical Microbiology Membrane Proteins - analysis Middle Aged Original Investigation Peripheral blood mononuclear cells Polymerase chain reaction Proto-Oncogene Proteins c-fos - analysis Proviruses - isolation & purification RAD51 gene Rad51 Recombinase - analysis Replication Signal transduction Statistical analysis T cell receptors Transformed cells Viral Load Virology Viruses
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Zusammenfassung:	Adult T cell leukemia/lymphoma (ATLL) is a life-threatening malignancy of HTLV-1 infected Th lymphocytes. In the present study host–virus interactions were investigated by assessment of HTLV-1 proviral load (PVL) and host gene expression. A cross-sectional study was carried out on 18 ATLL, 10 HAM/TSP patients and 18 HTLV-1 asymptomatic carriers (ACs). DNA and mRNA of the peripheral blood mononuclear cells were extracted for PVL and LAT, BIM, c-FOS and RAD51 gene expression measurement using qRT-PCR. The mean PVL in ATLL patients was 11,430 ± 3770 copies/10 4 which was statistically higher than ACs, 530 ± 119 copies/10 4 , ( p
ISSN:	0300-8584 1432-1831
DOI:	10.1007/s00430-017-0506-1