HPLC determination of tetracycline antibiotics in milk with post-column derivatization and fluorescence detection

The conditions for sensitive and selective determination of tetracyclines in milk in the form of their complexes with Mg 2+ via the HPLC method with post-column derivatization and fluorescence detection are found. It is shown that the fluorescence of tetracycline-Mg 2+ complexes in microemulsions is...

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Veröffentlicht in:	Inorganic materials 2016-12, Vol.52 (14), p.1365-1369
Hauptverfasser:	Kargin, I. D., Sokolova, L. S., Pirogov, A. V., Shpigun, O. A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetonitrile Analysis of Substances Antibiotics Chemistry Chemistry and Materials Science Fluorescence Industrial Chemistry/Chemical Engineering Inorganic Chemistry Inorganic materials Materials Science Microemulsions Milk Oxytetracycline Tetracyclines
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Zusammenfassung:	The conditions for sensitive and selective determination of tetracyclines in milk in the form of their complexes with Mg 2+ via the HPLC method with post-column derivatization and fluorescence detection are found. It is shown that the fluorescence of tetracycline-Mg 2+ complexes in microemulsions is 1.8 times more intense than that in aqueous acetonitrile medium. The detection limits of tetracycline, oxytetracycline, and doxycycline are 5, 8 and 25 ng mL –1 , respectively.
ISSN:	0020-1685 1608-3172
DOI:	10.1134/S0020168516140065