Unexpected effect of the vehicle (grain ethanol) of homeopathic FSH on the in vitro survival and development of isolated ovine preantral follicles

The aims of this study were to investigate the effects of medium replacement system (experiment I) and of FSH presentations (homeopathic – FSH 6cH and allopathic FSH – rFSH; experiment II) on the in vitro development, hormone production and gene expression of isolated ovine preantral follicles cultu...

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Veröffentlicht in:	Microscopy research and technique 2017-04, Vol.80 (4), p.406-418
Hauptverfasser:	Lima, Lartiza F., Rocha, Rebeca M. P., Duarte, Ana Beatriz G., Brito, Ivina R., Silva, Gerlane M., Rodrigues, Giovanna Q., Nunes‐Pinheiro, Diana C. S., Sales, Antônia D., Moura, Arlindo A., Wheeler, Matthew B., Rodrigues, Ana Paula R., Campello, Cláudio C., Figueiredo, José Ricardo
Format:	Artikel
Sprache:	eng
Schlagworte:	Androgens Animals Apoptosis - genetics Caspase 3 - analysis Cell Proliferation - drug effects Connexin 43 - analysis Connexins - analysis Culture DNA Fragmentation Estradiol - biosynthesis Ethanol Ethanol - chemistry Ethanol - pharmacology Ethyl alcohol Experiments Female Follicle Stimulating Hormone - pharmacology Gap Junction alpha-4 Protein Gene expression Grains Homeopathy hormone Hormones - biosynthesis Hormones - pharmacology in vitro culture In vitro testing Organ Culture Techniques - methods Ovarian Follicle - drug effects Ovarian Follicle - growth & development ovarian follicles Progesterone - biosynthesis Recombinant Recombinant Proteins - pharmacology Sex hormones Sheep Vehicles
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Beschreibung
Zusammenfassung:	The aims of this study were to investigate the effects of medium replacement system (experiment I) and of FSH presentations (homeopathic – FSH 6cH and allopathic FSH – rFSH; experiment II) on the in vitro development, hormone production and gene expression of isolated ovine preantral follicles cultured for 6 days. In experiment I, secondary follicles were cultured in the α‐MEM+ supplemented with FSH 6cH (0.05 fg/ml) or recombinant bovine FSH (100 ng/ml) without/with daily medium addition. The homeopathic FSH treatments with/without medium addition improved (p
ISSN:	1059-910X 1097-0029
DOI:	10.1002/jemt.22810