4-Phosphothiophen-2-yl alanine: a new 5-membered analogue of phosphotyrosine

4-Phosphothiophen-2-yl alanine: a new 5-membered analogue of phosphotyrosine

Polyclonal antibodies raised against 4-phosphothiophen-2-yl alanine 2a, a novel five-membered ring analogue of phosphotyrosine, showed high selectivity for phosphotyrosine and no cross-reactivity with other phosphorylated amino acids. Western blots showed that the polyclonal was similarly effective,...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2014-08, Vol.50 (66), p.9343-9345
Hauptverfasser: Lilley, Matthew, Mambwe, Bezaleel, Jackson, Richard F W, Muimo, Richmond
Format: Artikel
Sprache:eng
Schlagworte:
Alanine
Alanine - analogs & derivatives
Alanine - chemistry
Amino acids
Analogue
Antibodies
Blotting, Western
Monoclonal antibodies
Phosphorylation
Phosphotyrosine - chemistry
Selectivity
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Zusammenfassung:Polyclonal antibodies raised against 4-phosphothiophen-2-yl alanine 2a, a novel five-membered ring analogue of phosphotyrosine, showed high selectivity for phosphotyrosine and no cross-reactivity with other phosphorylated amino acids. Western blots showed that the polyclonal was similarly effective, but different in selectivity, to a commercially available monoclonal antibody.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc03393k