Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles
Biomolecules adsorbed on nanoparticles are known to confer a biological identity to nanoparticles, mediating the interactions with cells and biological barriers. However, how these molecules are presented on the particle surface in biological milieu remains unclear. The central aim of this study is...
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| Veröffentlicht in: | ACS nano 2017-02, Vol.11 (2), p.1884-1893 |
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| container_title | ACS nano |
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| creator | Lara, Sandra Alnasser, Fatima Polo, Ester Garry, David Lo Giudice, Maria Cristina Hristov, Delyan R Rocks, Louise Salvati, Anna Yan, Yan Dawson, Kenneth A |
| description | Biomolecules adsorbed on nanoparticles are known to confer a biological identity to nanoparticles, mediating the interactions with cells and biological barriers. However, how these molecules are presented on the particle surface in biological milieu remains unclear. The central aim of this study is to identify key protein recognition motifs and link them to specific cell-receptor interactions. Here, we employed an immuno-mapping technique to quantify epitope presentations of two major proteins in the serum corona, low-density lipoprotein and immunoglobulin G. Combining with a purpose-built receptor expression system, we show that both proteins present functional motifs to allow simultaneous recognition by low-density lipoprotein receptor and Fc-gamma receptor I of the corona. Our results suggest that the “labeling” of nanoparticles by biomolecular adsorption processes allows for multiple pathways in biological processes in which they may be “mistaken” for endogenous objects, such as lipoproteins, and exogenous ones, such as viral infections. |
| doi_str_mv | 10.1021/acsnano.6b07933 |
| format | Article |
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However, how these molecules are presented on the particle surface in biological milieu remains unclear. The central aim of this study is to identify key protein recognition motifs and link them to specific cell-receptor interactions. Here, we employed an immuno-mapping technique to quantify epitope presentations of two major proteins in the serum corona, low-density lipoprotein and immunoglobulin G. Combining with a purpose-built receptor expression system, we show that both proteins present functional motifs to allow simultaneous recognition by low-density lipoprotein receptor and Fc-gamma receptor I of the corona. 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However, how these molecules are presented on the particle surface in biological milieu remains unclear. The central aim of this study is to identify key protein recognition motifs and link them to specific cell-receptor interactions. Here, we employed an immuno-mapping technique to quantify epitope presentations of two major proteins in the serum corona, low-density lipoprotein and immunoglobulin G. Combining with a purpose-built receptor expression system, we show that both proteins present functional motifs to allow simultaneous recognition by low-density lipoprotein receptor and Fc-gamma receptor I of the corona. Our results suggest that the “labeling” of nanoparticles by biomolecular adsorption processes allows for multiple pathways in biological processes in which they may be “mistaken” for endogenous objects, such as lipoproteins, and exogenous ones, such as viral infections.</description><subject>Adsorption</subject><subject>Binding Sites</subject><subject>Biomolecules</subject><subject>Cells, Cultured</subject><subject>Coronas</subject><subject>Epitope Mapping</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Immunoglobulin G - chemistry</subject><subject>Immunoglobulins</subject><subject>Lipoproteins</subject><subject>Lipoproteins, LDL - chemistry</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Particle Size</subject><subject>Protein Corona - chemistry</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Receptors, IgG - chemistry</subject><subject>Receptors, LDL - chemistry</subject><subject>Recognition</subject><subject>Surface Properties</subject><issn>1936-0851</issn><issn>1936-086X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1LAzEQhoMotlbP3mSPgmybbJpkctTiR6FUEAVvSzY7q1u2SU12D_57t7T2JniaGXjed-Ah5JLRMaMZmxgbnXF-LAuqNOdHZMg0lykF-X582AUbkLMYV5QKBUqekkEGjGVa0CFZzkt0bV3V1rS1d4mvkhe0uGl9SO5qV9buI2l90n5if_q1b9B2jQnJzAfvzBZf9v83JrS1bTCek5PKNBEv9nNE3h7uX2dP6eL5cT67XaRmqniblkpIKkAJAQwQJJ_qaooKMrAWKAJwzTMEVjLMLChTFEZolNKiLQspMz4i17veTfBfHcY2X9fRYtMYh76LOQPNQUsJ4h-oZJIpLXmPTnaoDT7GgFW-CfXahO-c0XzrO9_7zve--8TVvrwr1lge-F_BPXCzA_pkvvJdcL2WP-t-AJzniuo</recordid><startdate>20170228</startdate><enddate>20170228</enddate><creator>Lara, Sandra</creator><creator>Alnasser, Fatima</creator><creator>Polo, Ester</creator><creator>Garry, David</creator><creator>Lo Giudice, Maria Cristina</creator><creator>Hristov, Delyan R</creator><creator>Rocks, Louise</creator><creator>Salvati, Anna</creator><creator>Yan, Yan</creator><creator>Dawson, Kenneth A</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0001-8870-5280</orcidid><orcidid>https://orcid.org/0000-0002-9339-0161</orcidid><orcidid>https://orcid.org/0000-0003-2938-4063</orcidid></search><sort><creationdate>20170228</creationdate><title>Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles</title><author>Lara, Sandra ; Alnasser, Fatima ; Polo, Ester ; Garry, David ; Lo Giudice, Maria Cristina ; Hristov, Delyan R ; Rocks, Louise ; Salvati, Anna ; Yan, Yan ; Dawson, Kenneth A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a473t-d756058755818e86349f4e7828cc80e883932e81d1e2c87abba59e66cecdb6623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adsorption</topic><topic>Binding Sites</topic><topic>Biomolecules</topic><topic>Cells, Cultured</topic><topic>Coronas</topic><topic>Epitope Mapping</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Immunoglobulin G - chemistry</topic><topic>Immunoglobulins</topic><topic>Lipoproteins</topic><topic>Lipoproteins, LDL - chemistry</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Particle Size</topic><topic>Protein Corona - chemistry</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Receptors, IgG - chemistry</topic><topic>Receptors, LDL - chemistry</topic><topic>Recognition</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lara, Sandra</creatorcontrib><creatorcontrib>Alnasser, Fatima</creatorcontrib><creatorcontrib>Polo, Ester</creatorcontrib><creatorcontrib>Garry, David</creatorcontrib><creatorcontrib>Lo Giudice, Maria Cristina</creatorcontrib><creatorcontrib>Hristov, Delyan R</creatorcontrib><creatorcontrib>Rocks, Louise</creatorcontrib><creatorcontrib>Salvati, Anna</creatorcontrib><creatorcontrib>Yan, Yan</creatorcontrib><creatorcontrib>Dawson, Kenneth A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>ACS nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lara, Sandra</au><au>Alnasser, Fatima</au><au>Polo, Ester</au><au>Garry, David</au><au>Lo Giudice, Maria Cristina</au><au>Hristov, Delyan R</au><au>Rocks, Louise</au><au>Salvati, Anna</au><au>Yan, Yan</au><au>Dawson, Kenneth A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles</atitle><jtitle>ACS nano</jtitle><addtitle>ACS Nano</addtitle><date>2017-02-28</date><risdate>2017</risdate><volume>11</volume><issue>2</issue><spage>1884</spage><epage>1893</epage><pages>1884-1893</pages><issn>1936-0851</issn><eissn>1936-086X</eissn><abstract>Biomolecules adsorbed on nanoparticles are known to confer a biological identity to nanoparticles, mediating the interactions with cells and biological barriers. However, how these molecules are presented on the particle surface in biological milieu remains unclear. The central aim of this study is to identify key protein recognition motifs and link them to specific cell-receptor interactions. Here, we employed an immuno-mapping technique to quantify epitope presentations of two major proteins in the serum corona, low-density lipoprotein and immunoglobulin G. Combining with a purpose-built receptor expression system, we show that both proteins present functional motifs to allow simultaneous recognition by low-density lipoprotein receptor and Fc-gamma receptor I of the corona. 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| source | MEDLINE; American Chemical Society Journals |
| subjects | Adsorption Binding Sites Biomolecules Cells, Cultured Coronas Epitope Mapping HEK293 Cells Humans Immunoglobulin G - chemistry Immunoglobulins Lipoproteins Lipoproteins, LDL - chemistry Nanoparticles Nanoparticles - chemistry Particle Size Protein Corona - chemistry Proteins Receptors Receptors, IgG - chemistry Receptors, LDL - chemistry Recognition Surface Properties |
| title | Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles |
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