A rapid screen for molecules that form duplex to duplex crosslinks in DNA

A rapid screen for molecules that form duplex to duplex crosslinks in DNA

We describe a gold nanoparticle based assay that can rapidly determine the crosslinking of DNA duplexes by ligands. Such compounds have potential in targeting highly compacted DNA such as that found in the nucleosome.

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2013-10, Vol.49 (80), p.9113-9115
Hauptverfasser: Marín, María J, Rackham, Benjamin D, Round, Andrew N, Howell, Lesley A, Russell, David A, Searcey, Mark
Format: Artikel
Sprache:eng
Schlagworte:
Aminacrine - chemistry
Assaying
Crosslinking
Deoxyribonucleic acid
DNA - chemistry
DNA - metabolism
Gold
Gold - chemistry
Hydrogen-Ion Concentration
Ligands
Metal Nanoparticles - chemistry
Nanostructure
Nucleosomes - metabolism
Screens
Spectrophotometry, Ultraviolet
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Beschreibung
Zusammenfassung:We describe a gold nanoparticle based assay that can rapidly determine the crosslinking of DNA duplexes by ligands. Such compounds have potential in targeting highly compacted DNA such as that found in the nucleosome.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc45600e