Nanochannel-based electrochemical assay for transglutaminase activity

Nanochannel-based electrochemical assay for transglutaminase activity

A novel electrochemical assay to quantify transglutaminase activity is reported. The assay is based on the enzyme-controlled diffusion of Fe(CN)6(3-/4-) through amino-functionalized nanochannels of a mesoporous silica thin film on a Au surface in the presence of N-benzyloxycarbonyl-L-glutaminylglyci...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2014-11, Vol.50 (87), p.13356-13358
Hauptverfasser: Fernández, Iñigo, Sánchez, Alfredo, Díez, Paula, Martínez-Ruiz, Paloma, Di Pierro, Prospero, Porta, Raffaele, Villalonga, Reynaldo, Pingarrón, José M
Format: Artikel
Sprache:eng
Schlagworte:
Assaying
Diffusion
Electrochemical Techniques
Enzyme Activation
Enzyme Assays - methods
Ferricyanides - chemistry
Ferricyanides - metabolism
Gold - chemistry
Gold - metabolism
Nanostructure
Nanostructures - chemistry
Particle Size
Porosity
Silicon dioxide
Silicon Dioxide - chemistry
Silicon Dioxide - metabolism
Surface chemistry
Surface Properties
Thin films
Transglutaminases - chemistry
Transglutaminases - metabolism
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Zusammenfassung:A novel electrochemical assay to quantify transglutaminase activity is reported. The assay is based on the enzyme-controlled diffusion of Fe(CN)6(3-/4-) through amino-functionalized nanochannels of a mesoporous silica thin film on a Au surface in the presence of N-benzyloxycarbonyl-L-glutaminylglycine.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc05083e