Application of Z‐sinapinic matrix in peptide MALDI‐MS analysis
Since introduction of sinapinic acid (SA) and α‐cyano‐4‐hydroxycinnamic acid as matrices, successful application of matrix‐assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite a...
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Veröffentlicht in: | Journal of mass spectrometry. 2017-03, Vol.52 (3), p.182-186 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Since introduction of sinapinic acid (SA) and α‐cyano‐4‐hydroxycinnamic acid as matrices, successful application of matrix‐assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E‐cinnamics. Here, Z‐SA as matrix for peptides is studied and compared with E‐SA and α‐cyano‐4‐hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright © 2017 John Wiley & Sons, Ltd. |
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ISSN: | 1076-5174 1096-9888 |
DOI: | 10.1002/jms.3908 |